从兰州急性腹泻儿童中检出人博卡病毒4型  

Discovery of Human Bocavirus 4 in Children with Acute Gastroenteritis in Lanzhou,China

作　　者：向静瑶[1] 马鑫[2] 郭延青 段招军 李宇宁[1] 李丹地 

机构地区：[1]兰州大学第一临床医学院儿科,甘肃730000 [2]宁夏医科大学检验学院,银川750004 [3]中国疾病控制预防控制中心病毒病预防控制所病毒性腹泻室,北京102206

出　　处：《病毒学报》2015年第1期42-45,共4页Chinese Journal of Virology

摘　　要：为了解兰州地区病毒性腹泻患儿中人博卡病毒4(HBoV4)的流行情况,收集兰州大学第一医院2012年7月至2013年6月5岁以下腹泻患儿的粪便标本331份,采用巢式PCR方法检测HBoV。将HBoV阳性序列与GenBank中相关序列进行比较分析,发现1例罕见HBoV4。该株与泰国株(GenBank登录号:JQ267789)核苷酸和氨基酸的同源性为98.9%、98.7%,与美国株(GenBank登录号:GQ506568)核苷酸和氨基酸的同源性为97.6%、97.4%,为典型的HBoV4,是我国首次在腹泻患儿粪便中发现。由进化树及同源性推测HBoV4可能存在两种不同的亚型。上述结果表明,我国首次在腹泻患儿粪便中检出HBoV4,且属于HBoV4两种可能亚型中的一种亚型。We studied the epidemiological characteristics of human bocavirus 4（HBoV4）in children with acute gastroenteritis in Lanzhou（China）.A total of 331 stool specimens were collected from children aged5years with acute diarrhea at the First Hospital of Lanzhou University between July 2012 and June2013.Specimens of HBoV were identified by nested polymerase chain reaction assays.Compared with related sequences in GenBank,the HBoV-positive strain isolated in the present study was,quite surprisingly,a rare genotype named HBoV4.This strain was a typical HBoV4,with high levels of nucleotide and amino acid homology to the Thailand strain,JQ267789（98.9% and 98.7%,respectively）,and the USA strain,GQ506568（97.6% and 97.4%,respectively）.This is the first report of HBoV4 as the causative agent for acute gastroenteritis in pediatric patients in China.This strain is one of two genotypes of HBoV that are currently circulating.

关 键 词：人博卡病毒4型 急性腹泻 基因型 

分 类 号：R373.9[医药卫生—病原生物学]