2013～2014年流感流行季节中国大陆B型流感病毒对NAI的敏感性  

Susceptibility of Influenza B Viruses to Neuraminidase Inhibitors Isolated during 2013-2014 Influenza Season in China's Mainland

作　　者：黄维娟[1] 李希妍[1] 谭敏菊[1] 隗合江[1] 成艳辉[1] 郭俊峰[1] 王钊[1] 肖宁[1] 王大燕[1] 舒跃龙[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所国家流感中心,卫生部医学病毒和病毒病重点实验室,北京102206

出　　处：《病毒学报》2015年第2期152-156,共5页Chinese Journal of Virology

基　　金："十二五"重大传染病专项课题"重要病毒的实验室网络化监测技术研究"(编号:2012ZX10004215-002)

摘　　要：为了解近期我国大陆流行的B型流感病毒对神经氨酸酶抑制剂类药物的敏感性,本文对2013年10月至2014年3月流感流行季节期间中国大陆分离的884株B型流感病毒进行了生物学耐药监测。884株B型流感毒株中所有毒株均对oseltamivir敏感,检测到2株(0.23%)对zanamivir敏感性降低的毒株,其他毒株均对zanamivir敏感。其中38株B/Victoria系毒株中B/Shandong-Kuiwen/1195/2014对zanamivir的IC50值为1.78nM,较药物敏感参考株的IC50平均值(0.34nM)高5.12倍,表明对zanamivir的敏感性降低,在NA基因上没有发现存在已知耐药位点变异,但有D35G,N59D和S402T(N2编码分别为39、64和399位)氨基酸位点的变异;846株B/Yamagata系毒株中B/Hunan-Lingling/350/2013对zanamivir的IC50值为2.72nM,较药物敏感参考株的IC50平均值(0.34nM)高7.99倍,表明对zanamivir的敏感性降低,NA基因发生了D197N(N2编码为198位)氨基酸位点的变异。根据结果可以推断目前我国流行的B型流感毒株对神经氨酸酶抑制剂类药物敏感。Data based on the antiviral-resistant phenotyping characteristics of 884 influenza B viruses circulating in China's Mainland from October 2013 to March 2014 were analyzed to assess the susceptibility of influenza B viruses to neuraminidase inhibitors.All 884 viruses were sensitive to oseltamivir;two viruses（0.23%）had reduced sensitivity to zanamivir and all other viruses were sensitive to zanamivir.Among the38 viruses with a B/Victoria lineage,B/Shandong-Kuiwen/1195/2014 exhibited a half-maximal inhibitory concentration（IC50）for zanamivir that was elevated by 5.12-fold（1.78nM）compared with neuraminidase inhibitors sensitive to the reference virus（0.34nM）,suggesting that it exhibited reduced inhibition by zanamivir.D35 G,N59Dand S402T（39,64 and 399with N2number）amino-acid substitutions in the NA gene were detected with no previously reported antiviral-resistant substitutions.Among viruses with the846B/Yamagata lineage,B/Hunan-Lingling/350/2013 exhibited a 7.99-fold elevated IC50 for zanamivir（2.72nM）compared with neuraminidase inhibitors sensitive to the reference virus（0.34nM）,suggesting that it exhibited reduced inhibition by zanamivir.D197N（N2number）,apreviously reported antiviral resistant-related amino-acid substitution in the NA gene,was detected in B/Hunan-Lingling/350/2013.These data suggest that recently circulating influenza B viruses in China's Mainland have retained susceptibility to neuraminidase inhibitors.

关 键 词：B型流感病毒 神经氨酸酶抑制剂 IC50值 敏感性 

分 类 号：R373.9[医药卫生—病原生物学]