小鼠卵巢玻璃化冻融过程中重组人卵泡刺激素干预对血管内皮生长因子(VEGF)及整合素αvβ3表达的影响  

Influence of recombinant FSH interpose on the vascular endothelial growth factor and integrin αvβ3 expression during cryopreservation by vitrification

作　　者：张红[1,2] 马文叶 杨延周[1] 裴秀英[1] 吴昊[1,2] 常青[2] 马会明[1] 马文智[1] 孙苗[1] 于佳[1] 蔡玉芳[2] 王红燕[1] 黑常春[2] 赵承军[2] 王燕蓉[1,2] 

机构地区：[1]宁夏医科大学生育力保持教育部重点实验室,宁夏生殖与遗传重点实验室,银川750004 [2]宁夏医科大学人体解剖与组织胚胎学系,银川750004

出　　处：《生殖与避孕》2015年第4期230-236,共7页Reproduction and Contraception

基　　金：国家自然科学基金项目,项目号:81160085,81260110

摘　　要：目的:观察重组人卵泡刺激素(r FSH)对小鼠卵巢血管内皮生长因子(VEGF)以及整合素αvβ3表达的影响。方法:将4周龄C57BL/6J小鼠卵巢随机分为新鲜对照组(FCG)、玻璃化冻存对照组(VCG)、300 IU/L r FSH干预玻璃化冻存组(r FSH-VG)。通过对冻融卵巢内各级卵泡计数、免疫组织化学观察VEGF和整合素αvβ3在卵巢不同细胞中的定位,Western blotting检测VEGF、整合素αv和整合素β3蛋白表达量。结果:各级卵泡计数、VEGF及整合素β3的蛋白表达均为r FSHVG组优于VCG组(P<0.05),整合素αv的表达组间均无统计学差异(P>0.05)。结论:玻璃化冻融过程中添加r FSH可以上调VEGF及整合素β3蛋白的表达。Objective： To investigate the influence of recombinant FSH （rFSH） interpose on the vascular endothelial growth factor （VEGF） and integrin αvβ3 expression during cryopreservation by vtrification. Methods： Four-week-old C57BL/6J mice in anestrus were chosen. Ovaries were randomized into fresh control group （FCG）, vitrification control group （VCG） and rFSH intervented vitrification group （rFSH-VG） treated by 300 IU/L rFSH during freezing-thawing. Follicles at different stages were counted; the expression ofVEGF, integdn αvβ3 in different cells of ovary were observed by immunohistochemical technique. The effect of rFSH intervention on the expression of VEGF, integrin αv, integrin β3 protein was observed and analyzed by Western blotting. Results： Follicle count, the expression of VEGF and integrin β3 were all higher in rFSH-VG group than in VCG group （P〈0.05）, the expression of integrin αv was not significantly different among those three groups （P〉0.05）. Conclusion： Vitrification freezing and thawing process of adding rFSH may increase the expression of VEGF and integrin β3 protein.

关 键 词：小鼠 卵巢 重组人卵泡刺激素(r FSH) 玻璃化冻存 血管内皮生长因子(VEGF) 整合素ΑVΒ3 

分 类 号：R714.8[医药卫生—妇产科学]