人精子-透明质结合试验与IVF-ET结局相关性研究  

Relationship between human sperm-hyaluronan binding assay and IVF-ET outcomes

作　　者：王金香[1] 邱孙全[1] 叶英辉[1] 金帆[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,杭州310006

出　　处：《生殖与避孕》2015年第4期247-251,281,共6页Reproduction and Contraception

基　　金：浙江省科技厅重大科技专项(编号:2011 C11033);浙江省重点科技创新团队自主申请项目(编号:2011R50013-22);国家科技支撑计划(编号:N20120266-04)

摘　　要：目的:分析精子-透明质结合试验(hyaluronan binding assay,HBA)与精子常规参数及体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)结局的相关性,探寻精子质量评估和授精方式选择的新指标。方法:对进入IVF-ET周期的136例患者进行HBA检测,并按HBA结合率将患者分为HBA结合率≥65%组(A组)与HBA结合率<65%组(B组),统计分析HBA与精液常规参数、体外受精率、胚胎质量和临床妊娠结局的相关性。结果:A组与B组相比,精子浓度无统计学差异(P>0.05);而前者的前向运动(PR)精子明显高于后者,差异有显著统计学意义(P<0.01)。A组总受精率和正常受精率均高于B组(P<0.01),而可利用胚胎率、临床妊娠率、胚胎着床率及活产婴儿出生率组间均无统计学差异(P>0.05)。结论:HBA反映精子活动力,对体外受精率具有指示作用,对于临床选择体外辅助授精方式具有一定的参考意义。Objective： To investigate the relationship between hyaluronan binding assay （HBA） and routine sperm parameters, to assess the potential value of HBA to predict fertilization and reproductive outcomes. Methods： A total of 136 patients undergoing IVF cycle for non-male infertility were divided into two groups based on HBA score cut-off （65%）. Routine sperm parameters, fertilization rate, embryo quality, implantation rate and reproductive outcome were compared between the two groups. Results： The rate of progressive （PR） sperm in group of HBA≥65% （group A） was significantly higher than that in group of HBA〈65% （group B） （P〈0.01）. Both total and normal fertilization rates were significantly higher in group A than those in group B （P〈0.01）. In addition, no relationship between HBA and available embryo rate, implantation rate, clinical pregnancy rate and live birth rate was found between the two groups （P〉0.05）. Conclusion： HBA is highly correlated with sperm motility and fertilization rate, thus, it may be a predictive parameter to select the method of fertilization.

关 键 词：精子-透明质结合试验(HBA) 体外受精(IVF) 受精率 临床妊娠 

分 类 号：R714.8[医药卫生—妇产科学]