黄芩苷对溃疡性结肠炎患者信号转导和转录激活因子表达的影响  

Effect of Baicalin on Signal Transduction and Activating Transcription Factor Expression in Ulcerative Colitis Patients

作　　者：于丰彦[1] 黄绍刚[2] 张海燕[2] 迟宏罡[1] 邹颖[1] 吕汝西[1,3] 郑学宝[1] 

机构地区：[1]广东医学院中医研究室,广东524023 [2]广东省中医院大学城分院脾胃科,广州511495 [3]南方医科大学中医药学院,广州510515

出　　处：《中国中西医结合杂志》2015年第4期419-424,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30772701);广东省东莞市科技计划项目(No.2008108101032)

摘　　要：目的研究黄芩苷对溃疡性结肠炎(ulcerative colitis,UC)患者信号转导和转录激活因子表达的干预作用。方法选取2010年6月—2011年1月广东省中医院大学城分院和南方医科大学南方医院消化内科门诊、住院部及消化内镜中心的患者作为研究对象,其中UC患者33例(UC组),腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)患者30例(IBS-D组),以30名健康体检者为健康对照组。取不同浓度的黄芩苷体外干预UC患者外周血单个核细胞。采用Q-PCR检测不同浓度黄芩苷体外干预下UC患者IL23R基因表达;应用Western blot法检测各组外周血单个核细胞信号转导和转录激活因子(STAT)4、STAT6及磷酸化(p)-STAT4、p-STAT6蛋白表达,ELISA法检测各组血清中IFN-γ、IL-4及IL-10水平变化,同时检测不同浓度黄芩苷对UC患者外周血单个核细胞蛋白表达及细胞因子IFN-γ、IL-4及IL-10水平的影响。结果与阴性对照组比较,浓度为40μmo L黄芩苷对US患者IL23R基因表达明显下降(P<0.01)。与健康对照组和IBS-D组比较,US组患者p-STAT4/STAT4值均增高(P<0.05),p-STAT6/STAT6值均下降(P<0.05),IFN-γ、IL-4及IL-10水平均升高(P<0.05)。UC患者在体外黄芩苷干预下,分别与阴性对照组、黄芩苷5、10μmo L组比较,浓度为20、40μmo L黄芩苷p-STAT4/STAT4值均下降(P<0.05),浓度为20、40μmo L/L黄芩苷pSTAT6/STAT6值均增高(P<0.05);浓度为20、40μmo L黄芩苷IFN-γ、IL-4水平降低(P<0.05),IL-10水平升高(P<0.05)。结论浓度为40μmo L黄芩苷的体外抑制p-STAT4/STAT4,调节p-STAT6/STAT6及相关细胞因子,使免疫平衡而缓解溃疡性结肠的炎症反应。Objective To explore the intervention of baicalin on signal transduction and activa- ting transcription factor expression of ulcerative colitis （UC） patients. Methods Recruited were UC pa- tients at Outpatient Department of Digestive Disease, Inpatient Department of Digestive Disease, Center for Digestive Endoscopy of College City Branch, Guangdong Provincial Hospital of Traditional Chinese Medicine, and Southern Hospital affiliated to Southern Medical University from June 2010 to January 2011. They were assigned to the UC group （33 cases） and the diarrhea-predominant irritable bowel syn- drome （IBS-D） group （30 cases）. Another 30 healthy subjects were recruited as a healthy control group. Peripheral blood mononuclear cells （PBMCs） in vitro intervened by different concentrations baicalin weretaken from UC patients. IL23R gene expressions in vitro intervened by different concentrations baicalin were detected using Q-PCR. Expressions of signal transducer and activator of transcription 4 （STAT4）, STAT6, phosphorylated-STAT4 （p-STAT4）, and p-STAT6 were detected using Western blot. Serum levels of IFN-γ, IL-4, IL-6, and IL-10 were measured by ELISA. Effects of different concentrations baicalin on ex- pressions of PBMCs, and levels of IFN-γ, IL-4, IL-10 of UC patients were also detected. Results Compared with the negative control group, 40 μmol baicalin obviously decreased IL23R gene expression of UC patients （P 〈0.01 ）. Compared with the healthy control group and the IBS-D group, p-STAT4/STAT4 ratios increased, p-STAT6/STAT6 ratios decreased, levels of IFN-γ, IL-4, IL-10 all increased in the US group （all P 〈0.05）. Compared with the negative control, 5 and 10μmol baicalin groups, 20 and 40 μmoL baicalin obviously decreased p-STAT4/STAT4 ratios （all P 〈0.05） ; 20 and 40 μmoL baicalin obviously in- creased p-STAT6/STAT6 ratios（all P 〈0.05）; 20 and 40 μmoL baicalin obviously lowered levels of IFN-γ and IL-4, and elevated IL-10 levels （all P 〈0.05）. Conclu

关 键 词：溃疡性结肠炎 信号转导和转录激活因子4 信号转导和转录激活因子6 磷酸化信号转导和转 录激活因子6 信号转导和转录激活因子6 黄芩苷 

分 类 号：R259[医药卫生—中西医结合]