不同环境温度对虚寒证和虚热证线粒体复合体Ⅱ活性的影响  

Effect of Various Ambient Temperatures on Activities of Mitochondrial Complex Ⅱ in Patients of Deficiency-cold Syndrome and Deficiency-heat Syndrome

作　　者：闫盼盼[1] 杨丽萍[1] 黄睿[1] 胡研萍[1] 侯俊林[1] 李新民[1] 詹向红[1] 

机构地区：[1]河南中医学院中西医结合基础学科,郑州450046

出　　处：《中国中西医结合杂志》2015年第4期434-437,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072734);河南省杰出青年基金项目(No.114100510011)

摘　　要：目的观察不同环境温度条件下虚寒证和虚热证线粒体复合体Ⅱ活性的变化规律。方法选择2012年11月在校的河南中医学院1～3年级本科生作为研究对象。通过中医体质量表和专家诊断筛选出虚寒证组（20例）、虚热证组（20例）及平和体质人群为健康对照组（20名）。采用分光光度法检测各组不同环境温度下线粒体复合体Ⅱ活性值。结果（1）各组不同温度下复合体Ⅱ活性比较：与本组室温条件下比较，健康对照组低温下复合体Ⅱ活性值升高，差异有统计学意义（P〈0．05），虚寒证组、虚热证组差异均无统计学意义（P〉0．05）；与健康对照组比较，虚寒证组低温、室温下复合体Ⅱ活性值均降低，差异有统计学意义（P〈0．05）；与虚寒证组比较，虚热证组在室温、低温下复合体Ⅱ活性值均升高，差异有统计学意义（P〈O．05）。（2）复合体Ⅱ活性调节速率变化：与本组室温条件下比较，健康对照组、虚热证组复合体Ⅱ活性低温下调节速率均升高，差异有统计学意义（P〈0．05），虚寒证组差异无统计学意义（P〉0．05）；与健康对照组比较，虚寒证、虚热证低温、室温下调节速率均降低，差异均有统计学意义（P〈0．05），与虚寒证组比较，虚热证组差异无统计学意义（P〉0．05）。结论环境温度可影响线粒体复合体Ⅱ活性，且对不同证型人群的影响程度不同，但其变化趋势基本一致。Objective To explore activity laws of mitochondrial complex Ⅱ in patients of defi- ciency-cold syndrome （DCS） and deficiency-heat syndrome （DHS） under various ambient tempera- tures. Methods Subjects were recruited by questionnaire and expert diagnosis from grade 1 -3 under- graduates at Henan College of Traditional Chinese Medicine in November 2012, and assigned to a normal control group, the DCS group, and the DHS group, 20 in each group. Their venous blood samples were collected at two different temperature conditions. Activities of mitochondrial complex Ⅱ were measured by spectrophotometry. Results （1） Comparison of mitochondrial complex Ⅱ under various ambient temperatures： Compared with room temperature in the same group, activity values were all increased in the normal control group at cold temperature with significant difference （P 〈0.05）, but there was no sig- nificant difference in the DCS group and the DHS group （P 〉0.05）. Compared with the normal control group, activity values of complex Ⅱ were reduced in the DCS group at cold and room temperatures with significant difference （P 〈0.05）. Compared with the DCS group, activity values of complex Ⅱ were in- creased in the DHS group with significant difference （P 〈0.05）. （2） Changes of adjustment rates ： Com- pared with room temperature, the adjustment rate all rose at cold temperature in the normal control group and the DHS group with significant difference （P 〈0.05）, but with no significant difference found in theDCS group （P 〉0.05）. Compared with the normal control group at the same temperature, the adjustment rate in the DHS group and the DCS group was all reduced at cold and room temperatures with significant difference （P 〈0.05）. There were no significant difference in the adjustment rate between the DHS group and the DCS group （P 〉 0.05）. Conclusions Environment temperature can affect the activity of mito- chondrial complex Ⅱ with different influence degrees on different

关 键 词：虚寒证 虚热证 复合体Ⅱ 能量代谢 

分 类 号：R241[医药卫生—中医诊断学]