熟地超滤膜提取物对氯化镉诱导骨髓间充质干细胞增殖及遗传稳定性变化的影响  

Effect of Ultrafiltration-membrane Extracts of Radix Rehmanniae Praeparata on Proliferation and Genetic Stability of Bone Marrow-Derived Mesenchymal Stem Cells Induced by Cadmium Chloride

作　　者：刘永琦[1,2,3] 张齐[1] 李静雅[1] 达瑞[1] 骆亚莉[1] 苏韫[1] 伍志伟[1] 颜春鲁[1] 聂蕾[1] 

机构地区：[1]甘肃中医学院甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730020 [2]甘肃省中药药理与毒理学重点实验室中西医结合基础室,兰州730020 [3]敦煌医学与转化省部共建教育部重点实验室,兰州730020

出　　处：《中国中西医结合杂志》2015年第4期450-456,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81360588);甘肃省高校科研基金项目(No.0906-03)

摘　　要：目的探讨熟地超滤膜提取物对氯化镉（CdCl2）诱导骨髓间充质干细胞（bonemarrowmes—enchymalstemcells，BMSCs）增殖及遗传稳定性变化的影响。方法采用微核实验、染色体分析、流式细胞术观察熟地超滤膜提取物对化学毒性物质CdCl2诱导BMSCs增殖、细胞微核率、染色体畸变率及凋亡率的影响。结果与CdCl2组比较，不同分子量段熟地超滤膜提取物在浓度为0．8g／L对CdCl2诱导的BMSCs有明显的促进增殖作用（P〈0．05）。与对照组比较，CdCl2组细胞微核率、染色体畸变率及凋亡率均明显增高（P〈0．05）；与CdCl2组比较，不同分子量段熟地超滤膜提取物对CdCl2诱导的BMSCs微核率、染色畸变率及凋亡率均明显降低（P〈0．05），其中BMSCs微核率及染色畸变率以10000分子量以下组降低最为明显（P〈0．05），凋亡率以10000-200000分子量组下降明显（P〈0．05）。结论熟地提取物可以减少CdCl2诱导BMSCs的凋亡率，降低BMSCs的微核率和染色体畸变率。Objective To study the effect of ultrafiltration-membrane extracts of Radix Reh- manniae Praeparata （UMERRP） on theproliferation and genetic stability of bone marrow-derived mesen- chymal stem cells （BMSCs） induced by cadmium chloride （CdCl2）. Methods Protective effects on the proliferation, micronuclear rates, chromosome aberration rates, and apoptosis rates were observed by micronuclei test, karyotype analysis, and flow cytometry. Results Compared with the CdCl2 group, UMERRP with different molecular weights at 0.8 g/L could obviously promote the proliferation （P 〈0.05）. Compared with the control group, micronuclear rates, chromosome aberration rates, and apoptosis rates were obviously enhanced in the CdCl2 group （P 〈0.05）. Compared with the CdCl2 group, UMERRP with different molecular weights could obviously decreased CdCl2 induced micronuclear rates, chromo- some aberration rates, and apoptosis rates （P 〈0.05）. Of them, BMSC micronuclear rates and chromo- some aberration rates decreased most obvious in UMERRP groups with molecular weight below 10 000 （P 〈0.05）. The apoptosis rate decreased most obviously in UMERRP groups with molecular weight ran- ging 100 000 and 200 000 （P 〈 0.05）. Conclusion UMERRP could reduce CdCl2 induced m icronuclearrates, chromosome aberration rates, and apoptosis rates.

关 键 词：骨髓间充质干细胞 氯化镉 熟地超滤膜提取物 微核率 染色体畸变率 凋亡 

分 类 号：R285[医药卫生—中药学]