温肾健脾法对腹泻型肠易激综合征大鼠血清T细胞亚群表达的影响  

Effect of Shen Warming Pi Strengthening Method on the Expression of Serum T Cell Subsets in IBS-D Rats

作　　者：苏晓兰[1] 魏茹涵 魏玮[1] 张静 白亦冰 史海霞[1] 

机构地区：[1]中国中医科学院望京医院脾胃病科,北京100102 [2]University of Toronto,Ontario M5S 1A1,Canada [3]北京市宣武中医医院脾胃病科,北京100050

出　　处：《中国中西医结合杂志》2015年第4期457-460,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然基金资助项目(No.81072800,No.81273746);国家自然基金青年基金项目(No.814033389)

摘　　要：目的观察温肾健脾法对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)大鼠血清T细胞亚群CD45+%、CD3+%及CD4+/CD8+表达的影响。方法参照AL-Chaer造模方法联合夹尾及番泻叶灌胃制备IBS-D大鼠模型,造模后30只SD大鼠按随机数字表法分为模型组、四神丸方组及温肾健脾方高、中、低剂量组,每组6只,同时设正常组SD大鼠6只。温肾健脾方高、中、低剂量组分别给予温肾健脾方免煎剂3.100、1.550、0.775 g/(kg·d)灌胃治疗,四神丸方组给予四神丸方免煎剂0.736 g/(kg·d)灌胃,同时正常组和模型组分别给予等体积生理盐水灌胃,持续灌胃2周。观察各组大鼠一般情况、血清T细胞亚群CD45+%、CD3+%及CD4+/CD8+表达情况。结果与正常组比较,模型组大鼠血清中CD45+及CD3+均升高,CD4+/CD8+值降低,差异均有统计学意义(P<0.05)。与模型组比较,温肾健脾方高、中、低剂量组及四神丸方组大鼠血清中CD45+及CD3+均降低,CD4+/CD8+值升高,差异均有统计学意义(P<0.05);与四神丸方组比较,除温肾健脾方低剂量组CD45+外,其他各指标差异均有统计学意义(P<0.05);与温肾健脾方低剂量组比较,温肾健脾方高、中剂量组及四神丸方组大鼠血清中CD3+均降低,四神丸方组及温肾健脾方高剂量组CD4+/CD8+值升高,差异均有统计学意义(P<0.05)。结论温肾健脾方对IBS-D大鼠有较好治疗作用,而温肾健脾法治疗IBS-D其机制可能是通过调控血清T细胞亚群CD45+%、CD3+%及CD4+/CD8+比值而起到调节免疫的作用。Objective To observe the effect of Shen warming Pi strengthening method on ex- pressions of serum T cell subsets （CD45＋%, CD3＋%, and CD4＋/CD8＋） in diarrhea-predominant irrita- ble bowel syndrome （IBS-D） rats. Methods An IBS-D rat model was established referring to AL-Chaer＇s modeling method combined with tail clamp and intragastric administration of sanna leaf. After modeling 30 SD rats were randomly divided into 6 groups according to random digit table, i.e., the model group, the high, middle, low dose Wenshen Jianpi Recipe （W JR） groups, and the Sishen Pill control group, 6 in each group. A normal control group consisting of 6 SD rats were also set up. Rats in high, middle, low dose W JR groups were administered by gastrogavage with boil-free WJR at the daily dose of 3.100, 1. 550, 0. 775 g/kg, respectively. Rats in the Sishen Pill control group were administered by gastrogavage with boil-free Sishen Pill at the daily dose of 0.736 g/kg. Equal volume of normal saline was given by gas- trogavage to rats in the model group and the normal control group. All medication lasted for 2 successiveweeks. Rats＇ general state, expressions of T cell subsets （0D45＋%, CD3＋%, and CD4＋/CD8 ＋） chan- ges were observed. Results Compared with the normal control group, expressions of CD45＋% and CD3＋% increased, but CD4＋/CD8＋ decreased with statistical difference （P 〈0.05）. Compared with the model group, expressions of CD45 ＋ % and CD3 ＋ % decreased, but CD4 ＋/CD8＋ increased with statistical difference in high, middle, low dose W JR groups, and the Sishen Pill control group （P 〈0.05）. Compared with the Sishen Pill control group, there was statistical difference in all indices except CD45 ＋ value in the low dose SWPSM group （P 〈0.05）. Compared with the low dose W JR group, the expression of CD3＋% decreased in high and middle dose W JR groups, and the Sishen Pill control group; CD4 ＋/CD8 ＋ increased in the Sishen Pill control group and the high dose SWPSM gro

关 键 词：腹泻型肠易激综合征 中医药疗法 温肾健脾法 T细胞亚群 

分 类 号：R285.5[医药卫生—中药学]