针刺不同穴位对大鼠顶核神经元自发放电的影响  

Effect of Acupuncture at Different Acupoints on Electric Activities of Rat Cerebellar Fastigial Nuclear

作　　者：梁超[1] 王媛 徐斌[1] 余芝[1] 

机构地区：[1]南京中医药大学第二临床医学院针药结合实验室,南京210046 [2]山东药品食品职业学院中药系,山东威海264210

出　　处：《中国中西医结合杂志》2015年第4期476-480,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(“973”)资助项目(No.2011CB505206);国家自然科学基金资助项目(No.81202744);国家自然科学基金资助项目(No.8133749);江苏省高校自然科学研究面上项目(No.11KJB360008);江苏省特色优势学科资助项目;江苏省高校青蓝工程优秀科技创新团队资助项目

摘　　要：目的通过观察针刺不同部位腧穴对小脑顶核(fastigial nucleus,FN)神经元自发放电的影响,探讨不同部位针刺信号是否传入小脑顶核及相应的效应特征。方法 120只SD雄性大鼠,在20%乌拉坦麻醉状态下定位右侧小脑顶核(坐标:AP 11.6 mm,RL 1.0 mm,H 5.6 mm),采用细胞外玻璃微电极记录的方法,记录该神经元自发放电作为基线。采用随机顺序依次针刺足三里、曲池、胃俞、中脘穴,与每次针刺前基线比较,观察对小脑顶核神经元放电的影响。结果与同穴位针刺前比较,针刺足三里、曲池、胃俞、中脘穴过程中FN神经元放电频率均增高(P<0.01,P<0.05)。中脘穴对FN放电反应率为56.00%(28/50),足三里、曲池、胃俞穴反应率分别为35.00%(21/60)、34.62%(18/52)、36.36%(20/55),中脘穴反应率明显高于其他穴位,差异有统计学意义(F=2.101,P<0.05)。结论针刺足三里、曲池、胃俞、中脘穴均可增高小脑顶核神经元自发放电频率,中脘穴对小脑FN具有优势调节作用。Objective To explore whether different acupuncture signals were afferent to the cer- ebellar fastigial nucleus （FN） neuron and to find out their corresponding effect features through observing the effect of spontaneous discharge of cerebellar FN neuron by needling at different acupoints. Methods Totally 120 male SD rats were anesthetized by 20% urethane and their right cerebellar FN were positioned （AP 11.6 mm,RL 1.0 mm,H 5.6 ram）. Extracelluar discharge was recorded by glass microelectrode （AP： -11.6 mm, R： 1.0 mm, H： 5.7 -7.0 mm）, using extracellular microelectrode recording method, recording the spontaneous discharge of cerebellar FN neurons as a baseline. Random order of needling at Zusanli （ST36）, Quchi （LI11 ）, Weishu （BL21）, and Zhongwan （CV12） were compared with the baseline before each acupuncture. Their effects on the discharge of cerebellar FN neurons were observed and compared with baselines. Results The frequency of FN neuronal discharge could be elevated by nee- dling at Zusanli （ST36）, Quchi （Llll）, Weishu （BL21）, and Zhongwan （CV12） （P 〈0.01, P 〈0.05）.The response rate of needling at Zhongwan （CV12, 56.00%） was higher than that of needling at Zusanli （ST36）, Quchi （LI11 ）, and Weishu （BL21） （35.00%, 34.62%, 36.63%, respectively ） with statistical difference （P 〈0.05）. The response rate of needling at Zhongwan （CV12） was obviously higher than that of needing at other points （F = 2.101, P 〈 0.05 ） Conclusions Needling at Zusanli （ ST36 ）, Quchi （Llll）, Weishu （BL21）, and Zhongwan （CV12） could elevate the spontaneous discharge frequency of cerebellar FN neurons. Needling at Zhongwan （CV12） had advantageous roles in regulating cerebellar FN.

关 键 词：针刺 胃运动 小脑顶核 神经元电活动 

分 类 号：R245[医药卫生—针灸推拿学]