天麻钩藤饮对自发性高血压大鼠血管功能及肾脏蛋白表达的影响  

Effect of Tianma Gouteng Decoction on the Endothelial Function and the Renal Protein Expression in Spontaneously Hypertensive Rats

作　　者：栗源 可燕[2] 蒋嘉烨[2] 李晓军[1] 江永波[1] 

机构地区：[1]上海中医药大学中药学院,上海201203 [2]上海中医药大学教学实验中心,上海201203

出　　处：《中国中西医结合杂志》2015年第4期481-487,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：上海市卫生局中医药科研基金资助项目(No.2012J008A);上海高校青年教师培养资助计划(No.shzy017)

摘　　要：目的探讨天麻钩藤饮对自发性高血压大鼠（spontaneouslyhypertensiverat，SHR）血管内皮功能和肾脏蛋白表达的影响及其可能机制。方法6周龄SHR18只，按照随机区组设计分为SHR空白组、天麻钩藤饮组与卡托普利组，每组6只。同时设同周龄WistarKyoto（WKY）大鼠6只为WKY对照组。天麻钩藤饮组给药量为10．26g，（kg·d），卡托普利组给药量为3．375g／（kg·d），大鼠6-24周龄每天灌胃1次，同时SHR空白组和WKY对照组按2mL／100g灌胃蒸馏水。各组大鼠每2周检测1次血压变化；采用血管环舒张度实验法测定肠系膜上动脉与胸主动脉舒张度以反映内皮功能；通过双向电泳分离肾脏总蛋白，Westernblot法验证显著差异蛋白。结果（1）与SHR空白组比较，卡托普利组（10-24周龄）血压下降明显（P〈0．01，P〈0．05）；天麻钩藤饮组在血压升高初期（10～12周龄）降压效果明显（P〈0．01），随着高血压进程的发展，降压效果不明显（P〉0．05）。（2）与SHR空白组比较，天麻钩藤饮组肠系膜上动脉血管舒张度得到明显改善（P〈0．05），WKY对照组肠系膜上动脉及胸主动脉血管舒张度明显优于SHR空白组（P〈0．01P〈0．05）；而卡托普利组各指标差异均无统计学意义（P〉0．05）。（3）肾脏蛋白的双向电泳分析：双向电泳发现16个显著差异肾蛋白，主要涉及一氧化氮系统、氧化应激及细胞骨架蛋白。Westernblot结果显示：天麻钩藤饮对Cu—Zn超氧化物歧化酶（Cu．ZnSOD）、4-α-氨甲喋呤脱水酶1（PCBDl）、精氨酸二甲基氨基水解酶2（DDAH2）的表达有明显改善作用（P〈0．05）。结论天麻钩藤饮对SHR大鼠肠系膜上动脉舒张功能具保护作用，其对高血压早期肾损伤的干预机制可能与调节一氧化氮系统与抗氧化应激相关。Objective To observe the effect of Tianma Gouteng Decoction （TGD） on the endothe- lial function and the renal protein expression of spontaneously hypertensive rats, and to analyze its possi- ble mechanism. Methods Totally 18 6-week-old SHR were randomly divided into 3 groups according to randomized block design, the SHR control group, the TGD group, and the captopril group, 6 in each group. Meanwhile, Wistar Kyoto （WKY） rats of the same age were recruited as a WKY control group. Rats in the TGD group were administered with TGD at the daily dose of 10. 260 g/kg. Rats in the captopril group were administered with captopril at the daily dose of 3.375 g/kg. 2 mL/100 g distilled water was administered to rats in the SHR control group and the WKY control group. All medication was performed by gastrogavage once per day till rats were 24 weeks old. Changes of blood pressure were measured once per two weeks. The relaxation of the thoracic aorta and the superior mesenteric artery was determined by vascular ring in vitro to reflect the endothelial function. The total renal protein was separated by two-dimensional electropho-resis （2-DE）. The significantly deviated protein was verified by Western blot. Results （1） Compared with the SHR control group, blood pressure was significantly lowered in rats （10 -24 weeks old） of the captopril group （P 〈0.01, P 〈0.05）. The hypotensive effect of TGD was obvious at the beginning of hypertension （10 -12 weeks） （P 〈0.01 ）. But along with the progression of hypertension, its hypotensive effect was not obvious （P 〉0.05）. （2） Compared with the SHR control group, the relaxation of the superior mesenteric ar- tery was obviously improved in the TGD group （P 〈0.05） ; the relaxation of the thoracic aorta and the supe- rior mesenteric artery was obviously superior in the WKY control group （P 〈0.01, P 〈0.05）. But there was no statistical difference in each relaxation index between the captopril group and the SHR control group （P 〉0.0

关 键 词：自发性高血压大鼠 天麻钩藤饮 双向电泳 血管内皮功能 

分 类 号：R285.5[医药卫生—中药学]