DCE-MRI评价Endostar对兔VX2骨肿瘤模型抗血管生成的疗效  

Feasibility of DCE-MRI for evaluation of anti-angiogenesis effect of Endostar in the model of rabbit VX2 bone tumor

作　　者：龚威[1] 查云飞[1] 闫力永[1] 邢栋[1] 王克军[1] 胡磊[1] 王娇[1] 刘昌盛[1] 

机构地区：[1]武汉大学人民医院放射科,武汉430060

出　　处：《放射学实践》2015年第4期313-318,共6页Radiologic Practice

基　　金：湖北省自然科学基金资助项目(2013CFB242);湖北省卫生厅科研资助项目(JX6B68)

摘　　要：目的:探讨基于Reference-Region模型的DCE-MRI评价恩度对兔VX2骨肿瘤抗血管生成疗效的可行性。方法:将成功建立胫骨VX2肿瘤模型(软组织肿块直径>1.0cm)的20只兔子随机分成对照组(n=10)和实验组(n=10),分别于给药前、连续给药14天后(对照组药物为生理盐水;实验组药物为恩度,药物浓度为1.5mg/8mL)行DCE-MRI扫描,使用Referrence Region模型获得肿瘤组织的对比剂容积转移常量Ktrans和反流速率常数Kep。于第14天扫描结束后处死动物,取肿瘤外周及中心区域的组织进行免疫组化染色检测,得到肿瘤不同区域的微血管密度(MVD)及血管内皮生长因子(VEGF)的表达情况,将DCE-MRI各参数与免疫组化结果进行相关分析。结果:对照组中肿瘤外周区域和中心区域的Ktrans值在治疗前分别为(32.58±3.10)和(28.50±3.54)min-1,治疗后分别为(37.66±2.78)和(34.20±3.39)min-1;实验组中肿瘤组织的外周区域和中心区域的Ktrans值在治疗前分别为(27.70±4.75)和(23.90±4.40)min-1,在治疗后分别为(22.20±4.29)和(18.30±4.23)min-1。实验组VX2骨肿瘤外周区域与中心区域的Ktrans值、MVD值及VEGF表达情况间的差异均有统计学意义(P<0.05),Kep值的差异无统计学意义(P>0.05)。实验组治疗后肿瘤外周和中心区域的VEGF表达、MVD值与Ktrans值间均呈正相关关系(r值分别为0.924、0.945、0.848和0.909,P<0.01);与Kep值间均无显著相关性(r值分别为0.022、0.162、0.219和0.042,P>0.01)。结论:兔VX2骨肿瘤模型的血流灌注空间分布具有异质性;基于Reference模型的DCE-MRI定量参数Ktrans可以评价恩度对兔VX2骨肿瘤抗血管生成的疗效。Objective：To explore the feasibility of dynamic contrast-enhanced magnetic resonance imaging （DCE-MRI）based on the Reference-Region model for evaluating anti-tumor angiogenesis effect of endostar in rabbit VX2 bone tumor.Methods：20 rabbits with VX2 bone tumor （tumor size in soft tissue〉1cm）were randomly divided into the control group （n= 10）and the experimental group （n= 10）,and were accepted DCE-MRI examination before and 14 days after treatment （using saline in control group;using endostar with concentration of 1.5mg/8mL in experimental group）.DCE-MRI parameters including microvascular permeability transfer constant （Ktrans ）and microvascular permeability reflux con-stant （Kep ）were acquired based on the Reference-Region model.All the rabbits were sacrificed after DCE-MRI scanning at the 14th day.MVD and VEGF expression were analyzed by immunohistochemical staining.Correlation analysis was per-formed between DCE-MRI parameters and immunohistochemistry results.Results：In the experimental group,the Ktrans , MVD and VEGF expression had statistical difference （P〈0.05）between peripheral region and central region of tumors, but the Kep value had no statistical difference （P〉0.05）between the two regions.Before treatment,the Ktrans value of pe-ripheral region and central region of rabbit VX2 bone tumors in the control group were （32.58±3.10）and （28.5± 3.54）min-1 respectively;and were （27.7±4.75）and （23.9±4.40）min-1 in the experimental group;after treatment,they were （37.66±2.78）and （34.2±3.39）min-1 in control group,and were （22.2±4.29）and （18.3±4.23）min-1 in the ex-perimental group,respectively.In the experimental group,the Ktrans values of the peripheral region and central region of rab-bit VX2 bone tumors were correlated with the MVD and VEGF expression （r= 0.924,0.945,0.848 and 0.909,respective-ly;P〈0.01）.The Kep values were not correlated with the MVD and the VEGF expression （r= 0.022,0.162,0.219 and 0.042,respectively;P

关 键 词：磁共振成像 动态对比增强扫描 骨肿瘤 恩度 抗肿瘤血管生成 

分 类 号：R445.2[医药卫生—影像医学与核医学] R738.1[医药卫生—诊断学]