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机构地区:[1]复旦大学神经生物学研究所,脑科学研究院,医学神经生物学国家重点实验室,上海200032
出 处:《生理学报》2015年第2期163-172,共10页Acta Physiologica Sinica
基 金:supported by the National Natural Science Foundation of China(No.31121061 and 30700218)
摘 要:大量研究表明多巴胺在行为决策、注意力调控和学习记忆等与前扣带皮层(anterior cingulate cortex,ACC)密切相关的认知功能中发挥重要作用。但是,多巴胺受体在ACC神经元上的分布还不清楚,尤其是在中间神经元上的分布。本研究旨在采用免疫组织化学和激光共聚焦扫描显微镜技术,研究多巴胺D1和D2受体在大鼠ACC一类主要类型的中间神经元,即表达钙结合蛋白的中间神经元上的分布。结果显示,D1和D2受体在ACC的小清蛋白(parvalbumin,PV),钙结合蛋白(calbindin D-28k,CB)及钙视网膜蛋白(calretinin,CR)阳性的中间神经元均有分布。其中,D1和D2受体在PV阳性中间神经元上表达较多,在CR阳性中间神经元上表达最少;D1和D2受体在ACC深层PV神经元上的表达比例显著高于浅层。此外,CR阳性中间神经元上D2受体分布比D1受体多。D1和D2受体的这种区域和中间神经元类型特异性的表达,为了解多巴胺对ACC功能的复杂调控提供了形态学基础。Dopamine plays an important role in cognitive functions including decision making, attention, learning and memory in the anterior cingulate cortex (ACC). However, little is known about dopamine receptors (DAR) expression patterns in ACC neurons, especially GABAergic interneurons. The aim of the present study was to investigate the expression of the most abundant DAR sub- types, D1 receptors (D1Rs) and D2 receptors (D2Rs), in major types of GABAergic interneurons in rat ACC, including parvalbumin (PV)-, calretinin (CR)-, and calbindin D-28k (CB)-containing interneurons. Double immunofluorescence staining and confocal scan- ning were used to detect protein expression in rat brain sections. The results showed a high proportion of PV-containing interneurons express D1Rs and D2Rs, while a low proportion of CR-positive interneurons express D1Rs and D2Rs. D1R- and D2R-expressing PV interneurons are more prevalently distributed in deep layers than superficial layers of ACC. Moreover, we found the proportion of D2Rs expressed in CR cells is much greater than that of D1Rs. These regional and interneuron type-specific differences of D1Rs and D2Rs indicate functionally distinct roles for dopamine in modulating ACC activities via stimulating D 1Rs and D2Rs.
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