子宫腔粘连患者子宫内膜组织中ER、PR的表达及意义  

Expression of estrogen receptor and progesterone receptor and their significance in the endometrial tissue of intrauterine adhesion patients

作　　者：任娟[1] 宋恩学[1] 王文艳[1] 卫兵[1] 

机构地区：[1]安徽医科大学第二附属医院妇产科,合肥230601

出　　处：《临床与实验病理学杂志》2015年第4期380-384,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金青年科学基金(81100412)

摘　　要：目的探讨雌激素受体(ER)、孕激素受体(PR)在子宫腔粘连(intrauterine adheious,IUA)患者子宫内膜组织中的表达及其临床意义。方法采用免疫组化Max Vision两步法和实时荧光定量PCR(qRT-PCR)技术检测IUA组(研究组)和非IUA组(对照组)子宫内膜组织中ER、PR的表达水平。结果免疫组化检测显示ER蛋白在研究组(3.52±0.71)和对照组(2.75±1.00)中的表达差异有统计学意义(P=0.01);qRT-PCR法检测显示ER mRNA在研究组(1.59±0.26)和对照组(1.00±0.19)中的表达差异有统计学意义(P=0.00)。免疫组化检测显示PR蛋白在研究组(3.26±0.70)和对照组(3.58±0.28)中的表达差异无统计学意义(P=0.12);qRT-PCR法检测显示PR mRNA在研究组(1.15±0.21)和对照组(1.00±0.31)中的表达差异无统计学意义(P=0.21)。免疫组化检测显示ER、PR蛋白分别在子宫内膜腺体和子宫内膜间质中均有表达,差异有统计学意义(χ2=5.797,P=0.016;χ2=4.857,P=0.027)。结论子宫内膜组织中ER表达研究组高于对照组,PR在两组之间的表达无差异;ER、PR蛋白在两组子宫内膜腺体中表达较多,在子宫内膜间质中表达较少甚至不表达;ER、PR表达的特点为IUA患者临床使用雌、孕激素治疗提供一定的理论参考。Purpose To investigate the expression of estrogen receptor （ER） and progesterone receptor （PR） in endometrial tissue in patients with intrauterine adhesion （IUA） and their clinical significance. Methods Two experimental methods, immunohiStochemieal MaxVision two step method and real-time fluoresceneequantitative PCR （ qRT-PCR）, were used for detection of ER and PR in endome- trial tissue both in group IUA （study group） and non IUA group （control group）. Results MaxVision immunohistoehemieal method showed that ER protein expression in the study group （3.52±0. 71 ） was significantly higher than that （2. 75± 1.00） in the control group （P=0. 01）, ER mRNA expression by qRT-PCR was also significantly higher in the study group （1.59 ± 0. 26） than that （ 1.00±0. 19） in the control group （ P = 0. 00）. The immunohistochemical detection of showed that PR protein expression in the study group （3.26±0. 70） had no significant different from that （3.58±0. 28） in the control group （P =0. 12）, qRT-PCR also showed that ER mRNA in the study group （ 1.15± 0. 21 ） had no significantly different from that （ 1.00 ±0. 31 ） in the control group （ P =0. 21 ）. Immunohistochemical expression of ER and PR proteins showed a significant difference between the endometrial glands and en- dometrial stroma （X^2 = 5. 797, P = 0. 016, X^2 = 4. 857, P = 0. 027）. Conclusion The expression of ER in endometrial tissue in the study group is higher than that in the control group, but the expression of PR has no different between the two groups, ER and PR pro- teins expression are higher in endometrial glands than that in endometrial stroma. These provide a theoretical reference for clinical use of estrogen and progesterone to IUA patients.

关 键 词：子宫腔粘连 ER PR 免疫组织化学 

分 类 号：R711.74[医药卫生—妇产科学]