戊四唑急性癫痫模型海马病理组织的变化  

Histopathological changes of hippocampus after acute epilepsy induced by pentylenetetrazole in rats

作　　者：刘小虎[1] 向绍杰[1] 齐越[1] 李淼[1] 李心培 孟莉[1] 陈贺[1] 贾冬[2] 

机构地区：[1]辽宁省中医药研究院,药理学教研室,辽宁沈阳110034 [2]辽宁中医药大学,辽宁沈阳110032

出　　处：《中国药理学通报》2015年第4期514-518,共5页Chinese Pharmacological Bulletin

基　　金：国家科技部“十二五”“重大新药创制”专项课题(No2012ZX09102201-005)

摘　　要：目的观察大鼠戊四唑急性癫痫模型造模后不同时间海马神经元损伤程度的变化。方法大鼠腹腔注射10 g·L-1(64 mg·kg-1)戊四唑1次,诱发大鼠急性癫痫发作,分别于注射戊四唑后24、72、120、144 h将大鼠麻醉,灌流取脑,采用尼氏染色及免疫组化染色观察大鼠海马神经元损伤程度。结果与空白对照组相比,腹腔注射戊四唑后海马神经元损伤程度随着时间的延长逐渐加重。结论戊四唑急性致痫模型大鼠海马神经元损伤的最大差异出现在腹腔注射戊四唑后120 h附近,可以将其作为药效学研究的海马组织取材时间。Aim To observe histopathological changes of hippocampus after acute epilepsy induced by pentylenetetrazole（ PTZ） in rats. Methods Five groups as control group,PTZ-induced 24 hours（ h） group,PTZinduced 72 hours group,PTZ-induced 120 hours group and PTZ-induced 144 hours group were designed. PTZ（ 64 mg·kg^-1） was administered with a single intraperitoneal injection for generalized tonic – clonic seizures in the current experiment. Control and PTZ treated animals were sacrificed after specific time points.Brain was dissected out and then evaluated for neuropathological changes using Nissl staining and immunohistochemical technique. Results In this study PTZinduced hippocampal neuron status apoptosis occurred at 24 hours and was sustained for 144 hours after status epilepticus. Whereas,activated caspase-3 and AIF appeared at 24 hours and were sustained for 144 hours after status epilepticus. Conclusion The results of this study show that the significant histopathological changes of hippocampus appear in the vicinity of 120 hours after intraperitoneal injection of pentylenetetrazole.

关 键 词：戊四唑 尼氏染色 CASPASE-3 AIF 癫痫 大鼠 

分 类 号：R332[医药卫生—人体生理学]