半乳凝素-9抑制胶质瘤细胞增殖及迁移  

Galectin-9 inhibits proliferation and migration of glioma cells

作　　者：姚众[1] 陈为亮[1] 徐洋洋[1] 何影[2] 曲迅[2] 李新钢[1] 

机构地区：[1]山东大学齐鲁医院神经外科山东大学脑科学研究所,山东济南250012 [2]山东大学齐鲁医院基础医学研究所,山东济南250012

出　　处：《山东大学学报（医学版）》2015年第4期1-5,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81172404;81372720)

摘　　要：目的研究半乳凝素-9(galectin-9)对胶质瘤细胞系U251增殖和迁移能力的影响,并探讨其作用机制。方法利用实时定量PCR方法检测胶质瘤细胞系中galectin-9的表达情况;加入不同浓度(1、4、8、16μg/m L)外源galectin-9蛋白处理一定时间(12、24、36 h)后,CCK-8、Transwell实验检测细胞增殖和迁移能力;另外,在galectin-9处理的同时加入其竞争性抑制剂乳糖,观察其对增殖、迁移作用的影响。结果 4种胶质瘤细胞系均极低水平表达galectin-9;8、16μg/m L浓度的galectin-9处理组的增殖率较对照组明显降低(P<0.001),随着时间延长,细胞增殖率逐渐降低(P<0.01),添加乳糖组细胞增殖率相对于未加乳糖组增高(P<0.05);外源性galectin-9刺激不同时间,穿至膜下的细胞数较对照组明显减少(P<0.01),添加乳糖组细胞数较未加乳糖组增多(P<0.05)。结论半乳凝素-9可明显抑制胶质瘤细胞的增殖,具有浓度和时间依赖性,并且能够有效抑制胶质瘤细胞的迁移。Objective To investigate the effects of exogenous galectin-9 on the proliferation and migration of glioma cells and its potential mechanism.Methods The expression of galectin-9 in glioma cells was detected with real-time quantitative polymerase chain reaction （PCR）.After glioma cell line U251 was treated with different concentrations of exogenous galectin-9 （1,4,8,16 μg /mL）,the changes of proliferation and migration were tested with cell count kit-8 （CCK-8）and transwell chambers respectively at different time （12,24,36 h）.When the competitive inhibitor lac-tose was added,the changes in proliferation and migration were detected.Results The expression of galectin-9 in 4 glioma cell lines was low or barely detected.Cells treated with 8 and 16 μg /mL galectin-9 showed decreased cell prolif-eration rates,compared with control group （P 〈0.001）.With time passing by,the cell proliferation rate decreased gradually （P 〈0.01）.After lactose treatment,cell proliferation rate increased （P 〈0.05）.Galectin-9 treatment signif-icantly reduced the numbers of cells penetrating the micropomus membrane （P 〈0.01 ）,while lactose treatment in-creased the number of cells,compared with the untreated group （P 〈0.05）.Conclusion Galectin-9 effectively inhib-its proliferation of glioma cells in a time-and dose-dependent manner,and it can also inhibit the migration of glioma cells.

关 键 词：半乳凝素-9 胶质瘤 增殖 迁移 

分 类 号：P739.4[天文地球—海洋科学]