蒙古黄芪根围土壤水浸液对自身种子发芽及生理特性的影响  

Effect of Water Extracts from Rhizosphere Soil of Cultivated Astragalus membranaceus var. mongholicus on It's Seed Germination and Physiological Characteristics

作　　者：郎多勇[1] 付雪艳[2,3,4] 荣佳旺 张新慧[2,3,4] 

机构地区：[1]宁夏医科大学实验动物中心,宁夏银川750004 [2]宁夏医科大学药学院 [3]宁夏回医药协同创新中心 [4]宁夏医科大学回医药现代化省部共建教育部重点实验室,宁夏银川750004

出　　处：《中药材》2015年第1期11-13,共3页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划子课题(2011BAI05B05)

摘　　要：目的:通过蒙古黄芪根围土壤提取液的生物学试验,研究蒙古黄芪前茬土壤提取液对自身种子萌发及幼苗生长的影响效应,探讨蒙古黄芪连作障碍与自毒作用的关系。方法:设置蒸馏水(CK)、蒙古黄芪根围土壤水浸提液50、100、200和400 mg/m L共5个处理,用于蒙古黄芪自毒作用的生物学试验。结果:当蒙古黄芪根围土壤水浸液浓度小于100 mg/m L时对自身种子出苗率、幼苗根长和活力指数均有显著的促进作用,而当根围土壤水浸液浓度大于200 mg/m L则对各指标没有显著影响效应。高于400 mg/m L的根围土壤水浸液显著抑制自身幼苗SOD活性;高于200 mg/m L的根围土壤水浸液显著抑制自身幼苗POD活性,而显著增加幼苗MDA含量。结论:较低浓度的蒙古黄芪根围土壤水浸液对自身种子萌发出苗和幼苗生长有一定的促进作用,而较高浓度则没有显著的影响效应,但对幼苗细胞膜造成了一定损伤。Objective： To explore the relationship between continuous cropping obstacle and autotoxicity of Astragalus membranaceus var. mongholicus. Methods： Distilled water（CK）,water extracts of rhizosphere soil（50,100,200 and 400 mg/m L） were applied to test their effect on early growth and physiological characteristics of Astragalus membranaceus var. mongholicus. Results： The water extracts from rhizosphere soil of cultivated Astragalus membranaceus var. mongholicus significantly increased seedling emergence rate,root length and vigor index of Astragalus membranaceus var. mongholicus seedling when at the concentration of 100 mg/m L or below,however,there was no significant effect at 200 mg/m L or higher. The water extracts from rhizosphere soil of cultivated Astragalus membranaceus var. mongholicus significantly reduced the SOD activity in Astragalus membranaceus var. mongholicus seedling at 400 mg/m L and POD activity at 200 mg/m L and 400 mg/m L,while significantly increased the MDA content. Conclusion： Water extracts from Astragalus membranaceus var. mongholicus rhizosphere soil significantly affected Astragalus membranaceus var. mongholicus germination and seedling growth in a concentration-dependent manner,generally,low concentrations increased the SOD and POD activity which improved seed germination and seedling growth,while high concentrations caused cell membrane damage of the seedling.

关 键 词：蒙古黄芪 根围土壤 水浸提液 抗氧化酶活性 膜脂过氧化 

分 类 号：R282.2[医药卫生—中药学]