东南亚进口燕窝中醛糖的气相色谱鉴定与含量测定  

Characterization of Aldoses in Edible Bird's Nest from Southeast Asia by Gas Chromatography

作　　者：赵斌[1,2] 邓仙梅 王羚郦[2] 李耿[2] 赖小平[2] 

机构地区：[1]中山火炬职业技术学院,广东中山528436 [2]广州中医药大学,广东广州510006 [3]广东药学院,广东广州510006

出　　处：《中药材》2015年第1期25-28,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81173498);国家国际合作专项(2014DFH30010)

摘　　要：目的:建立东南亚进口燕窝中4种醛糖的鉴定与含量测定方法。方法:供试品在2 mol/L的盐酸-甲醇溶液中水解20 h后,用氢氧化钾-甲醇溶液中和,再在吡啶-乙酸酐溶液中乙酰化。采用气相色谱法,HP-5毛细管柱,程序升温法进行测定。结果:燕窝GC图谱与伪品有明显区别。结论:该方法具有分辨率好、灵敏度高的特点,而且所获得的气相图谱具有很强的特征性、直观性和一致性,可作为燕窝的质量控制方法之一。Objective： To develop a method for the determination of aldoses in edible bird’ s nest（EBN） from Southeast Asia. Methods： Firstly,the sample was hydrolyzed by 2 mol/L HCl-methanol,neutralized by 2 mol/L potassium hydroxide methanol solution,and acetylated by acetic anhydride in pyridine. Then,four aldoses were separated completely with HP-5 capillary chromatogram column,by using FID tester,through programmed temperature. Results： The EBN’s GC spectrum was significantly different from its adulterants. Conclusion： The GC method is characteristic,simple and reliable,which can be used to control the quality of EBN.

关 键 词：燕窝 D-半乳糖 D-甘露糖 N-乙酰葡萄糖 N-乙酰半乳糖 气相色谱 

分 类 号：R284.1[医药卫生—中药学]