酒炙对大黄作用于上焦炎症及肝脏能量代谢的影响  

Effects of Wine-processing on Rhei Radix et Rhizoma on Upper-energizer Disease and Effects on Activities of Energy Metabolism Enzymes in Liver

作　　者：王亚[1,2,3] 芮天奇 杨军辉[1,2,3] 李俊松[1,2,3] 周玲玲[1] 蔡宝昌[1,3] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药高效给药系统工程技术研究中心,江苏南京210023 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023

出　　处：《中药材》2015年第1期53-57,共5页Journal of Chinese Medicinal Materials

基　　金：江苏省高校自然科学研究重大项目(10KJA360038)

摘　　要：目的:比较大黄酒炙前后对上焦炎症的药理作用差异,及其对机体肝脏能量代谢酶活性的影响。方法:制备大鼠醋酸灼烧创伤性口腔溃疡模型和大鼠肺炎链球菌性肺炎模型,灌胃给予大黄生、酒炙品,比较大黄酒炙前后对其病理改变的影响;小鼠灌胃给予大黄生、酒炙品,比较大黄酒炙前后对肝脏中Na+,K+-ATP酶、Ca2+-ATP酶、SDH酶(琥珀酸脱氢酶)活性的影响。结果:与生大黄比较,酒大黄可显著降低醋酸灼烧创伤性口腔溃疡大鼠炎症评分(P<0.05),对黏膜组织修复有更好的促进作用;显著降低肺炎链球菌性肺炎大鼠血中白细胞数和中性粒细胞比率,使其接近正常水平;显著降低TNF-α水平,与生品比较有显著性差异(P<0.05);可减少肺泡中炎症渗出情况。酒大黄对小鼠肝脏中能量代谢酶SDH(琥珀酸脱氢酶)、Ca2+-ATP酶、Na+,K+-ATP酶活性的抑制作用低于生大黄(P>0.05)。结论:酒炙后,大黄对上焦病症的治疗作用增强;对肝脏中能量代谢酶活性的抑制作用有减弱趋势。Objective： To compare the effects of crude and wine-processed Rhei Radix et Rhizoma on upper-energizer disease and hepatic energy metabolism in mice. Methods： The streptococcal pneumonia rats model and acetic acid burning mouth ulcers rats model were established and randomly divided into three groups： model group,crude Rhei Radix et Rhizoma group and wine-processed Rhei Radix et Rhizoma group. The pathologic changes were observed after the rats had been administrated with water extracts of crude and wineprocessed Rhei Radix et Rhizoma respectively. The normal ICR mice were randomly divided into three groups： control group,crude Rhei Radix et Rhizoma group and wine-processed Rhei Radix et Rhizoma group. The influence of water extracts of crude and wine-processed Rhei Radix et Rhizoma on the activities of Na＋,K＋-ATPase,Ca2 ＋-ATPase and succinic dehydrogenase（SDH） in the mice were compared. Results： Compared with the crude one,the wine-processed Rhei Radix et Rhizoma significantly decreased the inflammation scores（P 〈 0. 05）,and promoted the tissue repair of acetic acid burning mouth ulcers rats model. The wine-processed one could also obviously reduce and normalize the level of leucocyte and neutrophilic granulocyte,lower the TNF-α level（P 〈 0. 05）,and relieve inflammatory exudation of the lung tissue. The inhibitory effects of wine-processed Rhei Radix et Rhizoma on the activities of SDH,Ca2 ＋-ATPase and Na＋,K＋-ATPase were weaker than those of the crude one（P 〉 0. 05）. Conclusion： After having been processed with wine,the efficacy of Rhei Radix et Rhizoma on upper-energizer disease is enhanced,and the inhibition on the activity of energy metabolism enzyme in liver tends to be weakened.

关 键 词：大黄 酒炙 Na+ K+-ATP酶 CA2+-ATP酶 

分 类 号：R285.5[医药卫生—中药学] R283.1[医药卫生—中医学]