Inhibitory effect of chitosan on growth of the fungal phytopathogen,Sclerotinia sclerotiorum,and sclerotinia rot of carrot  

作　　者：WANG Qing ZUO Jin-hua WANG Qian NA Yang GAO Li-pu 

机构地区：[1]Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Fruits and Vegetable Storage and Processing/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China),Ministry of Agriculture/Key Laboratory of Urban Agriculture (North),Ministry of Agriculture

出　　处：《Journal of Integrative Agriculture》2015年第4期691-697,共7页农业科学学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (31101364);the Ministry of Agriculture of China (CARS-25-E-01 and 201203095);the Beijing Academy of Agriculture and Forestry Sciences,China (CXJJ201304)

摘　　要：The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.

关 键 词：antifungal activity CARROT CHITOSAN plasma membrane Sclerotinia sclerotiorum 

分 类 号：S436.31[农业科学—农业昆虫与害虫防治]