辽细辛精油不同馏分杀虫活性比较  

Comparison of the Biological Activities of Asarum Volatile Oil and Its Different Distillates

作　　者：王桂清[1] 李凡海 姬兰柱[2] 

机构地区：[1]聊城大学农学院,山东聊城252059 [2]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《湖北农业科学》2015年第2期355-358,共4页Hubei Agricultural Sciences

基　　金：山东省自然科学基金联合专项(ZR2012CL17);辽宁省科技计划项目(2012214001);沈阳市科技计划项目(F11-264-1-22);聊城大学重点建设项目(13KZ0801)

摘　　要：采用超临界CO2萃取辽细辛精油,硅胶柱层析法分离精油,气相色谱-质谱法分析其成分。以小菜蛾和淡色库蚊3龄幼虫为试虫,对辽细辛精油及各馏分的杀虫活性进行了比较。结果表明,各馏分的杀虫效果不同,馏分A5成分比较简单,但其杀虫活性并不高;其他馏分(如A1和A6)的组成成分较多,然而它们的杀虫活性却较高,说明辽细辛精油中起杀虫作用的主要成分并非一种,可能多种成分均起作用。该结果为进一步研究和开发以辽细辛为原料的新型植物源杀虫剂奠定理论基础。The asarum volatile oil was extracted with CO2 by supercutical fluid extraction（SFE） method and separated by silica column chromatography. GC-MS fingerprint chromatograms of different distillates were analyzed. The biological activities of the asarum volatile oil and different distillates were tested and compared by using the third instar larvae of Plutella xylostella and Culex pipiens pallens as the test insects. Results showed that the biological activities of different distillates were significant. The fifth distillate（A5） was unadulterated, but its biological activity was very low. Other distillates（for instance A1 and A6） were a mixture, but their biological activities were higher than that of A5. It is indicated that the main constituent of insecticidal action in asarum volatile oil was not onsdefold chemical compound. It is possible that many components in asarum volatile oil act on insecticide. It will provide a basis for developing new botanic insecticide using asarum as raw material.

关 键 词：细辛精油 GC-MS 硅胶柱层析 杀虫活性 

分 类 号：S482.3[农业科学—农药学]