斑马鱼SAA蛋白原核表达、抗体制备及菌结合试验  

Prokaryotic Expression, Antibody Preparation and Bacteria Combining of SAA Protein in Zebrafish

作　　者：齐麟[1] 李军 向志明[3] 余英才[4] 

机构地区：[1]铁道警察学院,郑州450053 [2]聊城大学药学院,山东聊城252000 [3]中国科学院南海海洋研究所,广州510301 [4]江西中医药大学生物化学教研室,南昌330004

出　　处：《湖北农业科学》2015年第2期382-384,共3页Hubei Agricultural Sciences

基　　金：有害生物控制与资源利用国家重点实验室开放项目(SKLBC2010K06);河南省高等学校青年骨干教师资助计划项目(2013GGJS-236)

摘　　要：通过构建斑马鱼(Danio rerio)SAA原核表达载体,在大肠杆菌(E.coli)TB1中诱导表达并纯化获得了斑马鱼SAA融合蛋白,将纯化的斑马鱼SAA蛋白免疫Balb/c小鼠,制备了特异性多克隆抗血清,并研究了斑马鱼SAA融合蛋白与细菌的结合能力及检测了抗体效价。结果表明,纯化的斑马鱼融合SAA蛋白能够与5种细菌直接结合,血清效价达到1∶2 000以上。The SAA fusion protein in zebrafish（Danio rerio） was expressed in E.coli TB1 and purified according to the construction of prokaryotic expression vector. The specific polyclonal antibodies were raised in Balb / c mouse immunized with the purified recombinant protein. The bacteria conjugation ability and the value of polyclonal antibodies were detected. The result s showed that the purified SAA protein in zebrafish was found to combine together with 5 bacteria. The value of obtained antibodies was up to 1∶2 000.

关 键 词：斑马鱼(Danio rerio) 血清淀粉样蛋白A 原核表达 抗体制备 菌结合 

分 类 号：Q789[生物学—分子生物学]