樱桃谷肉鸭H5N1亚型禽流感(Re-6株)灭活疫苗母源抗体消长规律及免疫程序研究  

Maternal Antibody Changes and Immune Procedure of H5N1 Subtype AI(Re-6 Strain) Inactivated Vaccine for Cherry Valley Meat Duck

作　　者：刘琨 

机构地区：[1]信阳市动物疫病预防控制中心,河南信阳464000

出　　处：《湖北农业科学》2015年第2期402-405,共4页Hubei Agricultural Sciences

基　　金：河南省科技厅科技攻关项目(122102110022)

摘　　要：选取1日龄健康无免疫的樱桃谷肉鸭480羽,随机分成A、B、C、D 4组,每组120羽。B、C和D组在10日龄分别免疫H5N1亚型禽流感(Re-6株)灭活疫苗0.5、0.8和1.0 m L/羽,A组为非免疫对照组。用HI方法检测其母源抗体和免疫抗体,根据母源抗体和免疫抗体消长规律,探讨樱桃谷鸭H5N1亚型禽流感(Re-6株)灭活疫苗的合理免疫程序。结果表明,樱桃谷鸭母源抗体效价和抗体合格率在1日龄最高,分别为6.6 log2和96.7%,14日龄下降至3.87 log2和56.7%,21日龄之后几乎没有保护力;3个试验组免疫抗体水平和抗体合格率在统计学上差异不显著(P>0.05),建议免疫程序为:10日龄免疫,免疫剂量0.8 m L/羽。480 one-day-old healthy non-immune Cherry Valley meat ducks were selected and randomly divided into four groups including A, B, C and D, and immunized with 0.0, 0.5, 0.8 and 1.0 m L H5N1 subtype AI（Re-6 strain） inactivated vaccine at 10 days old. Maternal antibody and immune antibody were examined with HI test. Based on their changes,rational immune procedure of H5N1 subtype AI（Re-6 strain） inactivated vaccine was established. The results showed that the titer of maternal antibody and the qualified rate of antibody tended to be highest at 1 days old, with 6.6 log2 and96.7%. At 14 days old, the indexes declined to 3.87 log2 and 56.7%. After 21 days old, the titer declined to nearly 0 log2.The levels and qualified rates of immune antibody in 3 groups showed no significant difference（P0.05）. It is indicated that the immune procedure might be at 10 days old with 0.8 m L.

关 键 词：H5N1高致病性禽流感 母源抗体 免疫程序 血凝抑制试验 HI抗体效价 抗体合格率 

分 类 号：S859.797[农业科学—临床兽医学]