漆酶水解对制浆造纸中段废水污泥亲油性的影响  

Influence of hydrolysis by laccase on pulp papermaking wastewater sludge and its lipophilicity

作　　者：韩卿[1,2] 钱威威[1,2] 刘睿[1,2] 李斯[1] 彭新文[2] 

机构地区：[1]陕西科技大学轻工与能源学院陕西省造纸技术及特种纸品开发重点实验室,陕西西安710021 [2]华南理工大学制浆造纸工程国家重点实验室,广东广州510641

出　　处：《陕西科技大学学报（自然科学版）》2015年第2期7-12,共6页Journal of Shaanxi University of Science & Technology

基　　金：华南理工大学制浆造纸工程国家重点实验室开放基金项目(201314)

摘　　要：对制浆造纸中段废水污泥的基础组分进行了分析.结果表明,该污泥含水率为78.11%,pH为5.43,有机物含量为46.74%,其中木质素含量为6.75%,粗纤维含量为27.34%.采用漆酶水解污泥后,其质量基本不变,而亲油性则得以明显提高.通过正交试验和单因素实验法探讨了漆酶预水解处理污泥的工艺条件,得出了适宜的反应时间、反应温度、酶用量和污泥浓度分别为4h、55℃、30U·g-1和130g·L-1,此时沉降时间为180min.对水解后污泥产物的红外光谱分析表明,在漆酶水解过程中,酶促反应使其大分子结构上的亲水基团,如酚羟基峰消失,从而提高了污泥的亲油特性.The base components of pulp and papermaking wastewater sludge were analyzed and listed as the water content was 78.11%,lignin content was 6.75%,crude fiber content was 27.34%,organic matter content was 46.74%and pH was 5.43.After enzymatic hydrolysis by laccase,the quality of sludge remains essentially unchanged,while the lipophilic performance can be significantly improved.The orthogonal and single factor experiments explored the pre-treatment conditions of laccase hydrolysis sludge process,the results showed that the suitable reaction time was 4h,reaction temperature was 55℃,the amount of enzyme was 30U·g-1 and the substrate concentrations was 130g·L-1.Under these conditions,the settling time was 180 min.The hydrolysate of sluge was analyzed by FTIR.The FTIR spectrum was indicated that the reason why the lipophilic performance of sluge was improved was that the enzymatic reaction leaded to the hydrophilic groups such as phenolic hydroxyl group disappear during laccase hydrolysis process.

关 键 词：造纸污泥 漆酶 亲油性 水解处理 

分 类 号：TS79[轻工技术与工程—制浆造纸工程] X793[环境科学与工程—环境工程]