芪卫颗粒对糖尿病肾病大鼠足细胞nephrin表达影响的实验研究  

Influence of Qiwei Granules on the expression of nephrin in podocyte of rats with diabetic nephropathy

作　　者：王馨瑶[1,2] 耿建国[1,2] 高彦彬[1,2] 李娇阳[1] 周盛楠[1] 邹大威[1] 刘迎新[1] 

机构地区：[1]首都医科大学中医药学院,北京硕士研究生100069 [2]中医络病研究北京市重点实验室

出　　处：《环球中医药》2015年第2期141-144,共4页Global Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2012CB518602);国家自然科学基金面上项目(81173238)

摘　　要：目的探讨芪卫颗粒对糖尿病肾病大鼠肾组织足细胞裂孔隔膜蛋白nephrin表达的影响。方法随机选取10只健康雄性SD大鼠为正常组,其余30只采用腹腔注射大剂量链脲佐菌素(60 mg/kg)诱导糖尿病大鼠模型,将成模大鼠随机分为模型组、西药组以及芪卫颗粒组,将西药组按缬沙坦10 mg/(kg·d)灌胃、芪卫颗粒组按生药10 g/(kg·d)灌胃,连续给药。于给药第24周检测大鼠血肌酐、血尿素氮及内生肌酐清除率;24周后将大鼠处死,采用PAS染色法将肾组织染色,在光镜下观察大鼠肾组织病理改变;采用western blot法检测肾组织足细胞裂孔隔膜蛋白nephrin表达情况。结果与正常组相比,模型组大鼠血肌酐、血尿素氮水平显著升高,内生肌酐清除率及足细胞nephrin表达水平显著下降(P<0.05);与模型组相比,各给药组大鼠血肌酐、血尿素氮水平明显下降,内生肌酐清除率及nephrin表达水平明显上调(P<0.05)。结论芪卫颗粒能够上调糖尿病肾病大鼠肾组织足细胞nephrin的表达,减轻足细胞损伤,保护肾功能,延缓糖尿病肾病的发生发展。Objective The study aims to investigate the effects of Qiwei Granules on the expression of nephrin in podocytes of rats with diabetic nephropathy（ DN）. Methods 10 normal male SD rats were chosen randomly as the normal group,and the other 30 rats were abdominally administered with a single injection of streptozotocin（ 60 mg / kg） to induce DN rat models. The model rats were divided into 3groups randomly： the model group,the western medicine group and Qiwei Granules group,Qiwei Granules group was given the prescription of Qiwei of 10 g /（ kg·d）,Western medicine group was given valsartan of 10 mg /（ kg·d） for 24 weeks. The changes in blood urea nitrogen,serum creatinine and urinary were observed in the 24 thweek. After 24 weeks,all the rats were sacrificed. The pathological change of renal tissue was detected by PAS. And the expression of nephrin in podocyte was observed by western blot. Results Compared with normal control group,the renal function and the expression of nephrin in model groupwere significantly decreased（ P〈0. 05）. Compared with model group,the renal function and the expression of nephrin in other groups were significantly increased（ P〈0. 05）. Conclusion The taking of Qiwei Granules could up-regulate the expression of nephrin in podocyte,decrease the damage of podocyte,and improve the renal function,which may slow the progression of DN.

关 键 词：芪卫颗粒 糖尿病肾病 足细胞 NEPHRIN 

分 类 号：R285.5[医药卫生—中药学]