连夏配方颗粒对心梗后大鼠心肌组织酪氨酸羟化酶mRNA、乙酰胆碱酯酶mRNA表达的影响  

Effects of Lian Xia Formula Granule on TH mRNA and Ach E mRNA expression level in rats after myocardial infarction

作　　者：李家立[1] 李平[2] 马洪皓[3] 田辰[1] 郝腾[1] 白芳芳[2] 刘卫宁[2] 马娜娜[1] 

机构地区：[1]北京中医药大学,博士研究生100029 [2]北京中医药大学第三附属医院心内科 [3]北京中医药大学第三附属医院心内科重症监护室

出　　处：《环球中医药》2015年第2期162-165,共4页Global Traditional Chinese Medicine

基　　金：国家科技支撑计划(2007BAI10B02-07);北京中医药大学自主选题(2013-ZYLC-015);北京中医药大学自主选题(2013-JYBZZXS-170)

摘　　要：目的观察连夏配方颗粒对心肌梗死后大鼠心梗周围区心肌组织酪氨酸羟化酶(tyrosine hydroxylase,TH)mRNA与乙酰胆碱酯酶(acetylcholinesterase,Ach E)mRNA表达的影响。方法将90只健康雄性成年Wistar大鼠随机分为假手术组、模型组、美托洛尔组、连夏配方颗粒189.00 mg/kg、94.50 mg/kg、47.25 mg/kg组;假手术组只穿线,不结扎左冠状动脉前降支,余各组均结扎左冠状动脉前降支以建立急性心肌梗死大鼠模型;假手术与模型组大鼠予生理盐水灌胃,余各组均予相应实验药物;连续给药30天后,采用Real-time RT-PCR方法检测心梗周围区心肌TH mRNA与Ach E mRNA表达水平。结果与假手术组比较,模型组大鼠心梗周围区心肌组织TH mRNA与Ach E mRNA表达均明显增高(P<0.05或P<0.01);与模型组比较,连夏配方颗粒189.00、94.50、47.25 mg/kg组大鼠心梗周围区TH mRNA与Ach E mRNA表达均明显降低(P<0.05或P<0.01);与美托洛尔组比较,连夏配方颗粒189.00 mg/kg组大鼠心梗周围区TH mRNA与Ach E mRNA表达均明显降低(P<0.05或P<0.01)。结论连夏配方颗粒可显著降低心肌梗死后大鼠心梗周围区心肌组织TH mRNA与Ach E mRNA的表达,达到改善心肌梗死后大鼠心梗周围区自主神经重构的作用。Objective To investigate the effects of Lian Xia Formula Granule（ LXFG） on TH mRNA and Ach E mRNA expression level at infarcted border zone of rats after myocardial infarction（ MI）.Methods Ninty healthy adult male Wistar rats were randomly assigned to six groups： sham operated group,model group,metoprolol group,189. 00 mg / kg-dose of LXFG group,94. 50 mg / kg-dose of LXFG group、47. 25 mg / kg-dose of LXFG group. The sham operated rats were not ligated in the left anterior descending coronary artery except the rest groups. The sham operated group and model group were given with normal saline by intragastric administration,and the rest groups were given with medicine for testing by intragastric administration. Thirty days later,TH mRNA and Ach E mRNA expression level were separately measured in all groups. Results Compared with the model group,TH mRNA and Ach E mRNA expression level in the 189. 00,94. 50,47. 25 mg / kg-dose of LXFG group decreased（ P〈0. 05 or P〈0. 01）. Com-pared with the metoprolol group,TH mRNA and Ach E mRNA expression level in the 189. 00 mg / kg-dose of LXFG group decreased（ P〈0. 05 or P〈0. 01）. Conclusion Application of Lian Xia Formula Granule in rats after MI can significantly decrease the expression level of TH mRNA and Ach E mRNA to improve cardiac autonomic neural remodeling.

关 键 词：连夏配方颗粒 心肌梗死 自主神经重构 酪氨酸羟化酶 乙酰胆碱酯酶 

分 类 号：R285.5[医药卫生—中药学]