糖肾宁对糖尿病肾病大鼠肾组织nephrin、desmin表达的影响  

Effects of Tangshenning on nephrin,desmin expression of renal tissue in STZ-Induced diabetic rats

作　　者：邹大威[1,2] 高彦彬[1,2] 李娇阳[1,2] 周盛楠[1,2] 王馨瑶[1,2] 龚慕辛[1,2] 耿建国[1,2] 王金羊[1,2] 朱智耀[1,2] 张娜[1,2] 

机构地区：[1]首都医科大学中医药学院,100069 [2]首都医科大学中医药学院中医络病研究北京市重点实验室,100069

出　　处：《环球中医药》2015年第3期307-312,共6页Global Traditional Chinese Medicine

基　　金：北京市教育委员会首都中医药研究专项面上项目(13ZY07)

摘　　要：目的探讨糖肾宁对链脲佐菌素诱导的糖尿病大鼠肾小球足细胞损伤的影响。方法8周龄SPF级雄性SD大鼠46只,随机选择10只为对照组,其余大鼠予大剂量STZ腹腔注射建立糖尿病模型。其中34只大鼠血糖≥16.7 mmol/L视为糖尿病模型建立成功,随机分为模型组、缬沙坦组、糖肾宁组,予糖肾宁及缬沙坦干预12周,测定空腹血糖、血清尿素氮、血清肌酐、24小时尿蛋白,足细胞裂孔隔膜蛋白nephrin、骨架蛋白desmin的蛋白或基因表达。结果与对照组比较,模型组大鼠空腹血糖、血清尿素氮、血清肌酐、24小时尿蛋白均明显升高(P<0.05),desmin蛋白、基因表达上调(P<0.05)、nephrin mRNA表达下调(P<0.05);与模型组比较,糖肾宁、缬沙坦干预后,desmin蛋白、基因表达下调(P<0.05)、nephrin mRNA表达上调(P<0.05),血清尿素氮、血清肌酐、24小时尿蛋白显著降低(P<0.05),但血糖无明显变化。结论糖肾宁干预链脲佐菌素诱导的糖尿病肾病大鼠可减少肾小球足细胞损伤,降低蛋白尿、保护肾功能。Objective Objective To investigate the effects of Tangshenning on podocyte injury of renal tissue in streptozotocin （STZ） induced diabetic rats. Methods：Non-diabetic rats （n=10） were designed as the control group, other rats were given STZ to induce diabetic model. The diabetic rats （ fasting blood glucose≥16.7mmol /L）were randomly divided into three groups： the model group（n =12）, the Tangshenning group （n=11） and the Valsartan group（n=11）, After intervention with Tangshenning and valsartan for 12 weeks, 24h Urinary protein excretion （24h UPro）, fasting blood glucose （FBG）, serum creatinine （ Scr） , serum urea nitrogen（ BUN） were examined. The protein and mRNA expression of podo-cyte slit diaphragm protein nephrin and cytoskeletal protein desmin mRNA expression were accessed. Results FBG, BUN, SCr, 24h UPro as well as desmin protein and mRNA expression of renal tissue in model group were increased significantly （ P〈0. 05 ） , nephrin mRNA expression of renal tissue in model group were decreased significantly （ P 〈0. 05 ） compared with non-diabetic rats group. Obviously, after Tangshenning or Valsartan intervention, 24h UPro, BUN and SCr were significantly declined（P〈0. 05）, while no significant change of FBG after intervention （P〉0. 05）. Tangshenning or valsartan could also decrease desmin expression and increase nephrin expression compared with model group. Conclusion Tan-gshenning could alleviate podocyte injury of renal tissue in STZ-induced diabetic rats, as well as reducing proteinuria and protecting renal function.

关 键 词：糖尿病肾病 足细胞损伤 糖肾宁 

分 类 号：R285.5[医药卫生—中药学]