水相分子印迹光子晶体水凝胶传感器检测尿液中的痕量吗啡  

Water-compatible Molecularly Imprinted Photonic Hydrogels for Fast Screening of Morphine in Urine

作　　者：孟梁[1] 孟品佳[2] 张庆庆[2] 王彦吉[2] 

机构地区：[1]福建警察学院刑事科学技术系,福州350007 [2]中国人民公安大学刑事科学技术学院,北京100038

出　　处：《分析化学》2015年第4期495-501,共7页Chinese Journal of Analytical Chemistry

基　　金：北京市与中央在京高校共建项目科学研究与研究生教育培养共建项目;福建省中青年教师教育科研项目(JA14336)资助

摘　　要：结合水相分子印迹和光子晶体技术构建了吗啡分子印迹光子晶体水凝胶传感器,并成功用于生物样品中痕量吗啡的筛查。以吗啡为印迹模板,甲基丙烯酸为单体,乙二醇二甲基丙烯酸甲酯为交联剂,填充至二氧化硅光子晶体模板孔隙中进行共价型分子印迹聚合,在1%HF溶液中除去光子晶体模板,并洗脱印迹模板分子,即可得到具有目标分子传感功能的分子印迹光子晶体水凝胶传感器。此传感器在水相环境中对吗啡分子的识别能力良好,可以在不需要标记的情况下,将目标分子的识别转变为衍射峰的位移,该光学信号通过传感器颜色的变化表现出来,吗啡浓度由10 pg/m L增加到1μg/m L过程中,衍射峰最大偏移达到38 nm,并且抗干扰能力强,检出限为0.1μg/L,响应时间为40 s,可以重复使用。此检测平台不需要对样品进行处理,便可准确、灵敏、快速地检测复杂样品中的目标分析物。A smart label-free colorimetric chemosensor was developed for the convenient and fast screening of morphine with high sensitivity and specificity based on colloidal-crystal and molecular imprinting technique which was named molecularly imprinted photonic hydrogels（ MIPHs）. The voids of photonic crystal template were completely filled with hydrogel with target template molecule. Then the silica particles and template molecule in it were fully etched to form 3D ordered interconnected macroporous arrays structure. Due to the unique inverse opal arrays with a thin polymer wall in which the imprinted nanocavities of morphine molecules distributed,the photonic hydrogels would be obvious swelling in responsive for the specific morphine molecular recognition process to induce the Bragg diffractive shifts of ordered macroporous arrays,which transferred into visually perceptible optical signal（ change in color） directly detected by the naked eye. The MIPHs were designed as water-compatible and synthesized in a water-methanol system to enhance the recognition ability in aqueous media. The proposed MIPHs were successfully employed to screen trace level morphine in human urine samples,exhibited high sensitive（ as low as 0. 1 ng / m L）,rapid responsive（ less than 40 s） and specific detection of morphine in complex biological matrix. It can provide a powerful tool for fast screening of morphine on the spot for forensic investigations.

关 键 词：水相分子印迹 光子晶体 化学传感器 吗啡 快速检测 尿样 

分 类 号：O657.3[理学—分析化学] R446.12[理学—化学]