当归芍药散及其拆方对肝硬化腹水大鼠电解质的影响  

Effect of Danggui Shaoyao Powder and Its Separated Prescription on Electrolytes in Rats with Cirrhotic Ascites

作　　者：姚瑶[1] 王成业[1] 岳晓莉[1] 潘永福[1] 王运来[1] 许钒[1] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230012

出　　处：《安徽中医药大学学报》2015年第2期56-59,共4页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81173368)

摘　　要：目的观察当归芍药散及其拆方对肝硬化腹水大鼠电解质紊乱的纠正作用。方法将雄性SD大鼠随机分为正常组,模型组,甘利欣组,当归芍药散低、中、高剂量组,拆方活血药组和利水药组。采用苯巴比妥联合四氯化碳腹腔注射复制肝硬化腹水大鼠模型,用代谢笼收集大鼠24h尿液,采用冰点法测定大鼠尿渗透压,采用全自动生化分析仪检测大鼠血清及尿K+、Na+、Cl-浓度。结果与模型组比较,当归芍药散各剂量组大鼠尿渗透压显著降低(P<0.05),血清K+、Na+、Cl-水平显著升高(P<0.05),尿K+、Na+水平显著降低(P<0.05);当归芍药散各剂量组与甘利欣组、活血药组、利水药组血清和尿电解质水平比较,差异无统计学意义(P>0.05)。结论当归芍药散可纠正肝硬化腹水大鼠电解质紊乱,无明显剂量依赖性,全方和拆方的效应无明显差异。Objective To investigate the effect of Danggui Shaoyao Powder and its separated prescription on the electrolyte disturbance in rats with cirrhotic ascites.Methods Male Sprague-Dawley rats were randomly divided into normal group,model group,Ganlixin group,low-,middle-,and high-dose Danggui Shaoyao Powder groups,blood-activating separated prescription group,and diuresis-promoting drug group.A rat model of cirrhotic ascites was established by intraperitoneal injection of phenobarbital combined with carbon tetrachloride.Metabolic cages were used to collect rat urine within 24hours;urine osmotic pressure was measured by cryoscopy,and the levels of K^＋,Na^＋,and Cl^-in serum and urine were determined by the automatic biochemical analyzer.Results Compared with the model group,all Danggui Shaoyao Powder groups had significantly reduced urine osmotic pressure（P〈0.05）,significantly increased serum levels of K^＋,Na^＋,and Cl^-（P〈0.05）,and significantly reduced urine levels of K^＋and Na^＋（P〈0.05）.There were no significant differences in serum and urine levels of electrolytes between the Danggui Shaoyao Powder group and the Ganlixin group,blood-activating separated prescription group,or diuresis-promoting drug group（P〉0.05）.Conclusion For rats with cirrhotic ascites,Danggui Shaoyao Powder can correct electrolyte disturbance with little dose dependence,and its effect shows no significant difference between the whole prescription and the separated prescription.

关 键 词：肝硬化腹水 电解质紊乱 当归芍药散 活血利水 

分 类 号：R285.5[医药卫生—中药学]