配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究  

Study on removing ginkgolic acids from EGb by coordination-organic solvent method

作　　者：杨世龙[1] 黄春丽[2] 唐颖[3] 徐莉[2,3,4] 蒋国斌[5] 曹福亮[4,5] 雷萌[2] 陈泳[2] 王燕芹[2] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037 [2]南京林业大学理学院,江苏南京210037 [3]南京林业大学现代分析测试中心,江苏南京210037 [4]南京林业大学南方现代林业协同创新中心,江苏南京210037 [5]南京林业大学林学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2015年第2期7-13,共7页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业公益性行业科研专项项目(201004015;20120460102);江苏省第四期"333高层次人才培养工程"科研项目(BRA2012171);"十二五"国家科技支撑计划(2012BAD21B04);南京林业大学优秀博士学位论文创新基金项目

摘　　要：银杏叶提取物(Extracts of Ginkgo biloba,EGb)具有诸多生物活性,但银杏酚酸的存在限制了EGb的应用。目前,去除EGb中银杏酚酸的方法主要有有机溶剂法和柱层析法,笔者在有机溶剂法的基础上提出配位-有机溶剂法,即在有机溶剂法最佳工艺条件的基础上引入金属离子配位以增加去除银杏酚酸的效果。结果表明:利用配位-有机溶剂法可以有效去除银杏酚酸,并有效减少有机溶剂洗涤次数,且不影响总银杏黄酮苷和总银杏内酯的含量;同时,EGb中的部分黄酮与锌离子形成配合物,可以提高EGb的抗肝癌细胞(HepG2)的活性。Extracts of Ginkgo biloba（EGb） has many biological activities,however,the existence of ginkgolic acids in EGb limit the applications of EGb. Currently,organic solvent method and column chromatography method are the main ways to remove ginkgolic acids from EGb. As a new method,coordination-organic solvent method was put forward based on organic solvent method. In coordination-organic solvent method,metal ions were added to the mixed solution from the best technological conditions of organic solvent method. The results showed that the coordination-organic solvent method could not only effectively remove ginkgolic acids,but also reduce the extraction times without affecting the content of total flavonoid glycosides and total ginkgolides. Meanwhile,a small amount of flavonoids and zinc ions could form complexes,which could improve the EGb activity of anti-hepatoma cells（HepG2）.

关 键 词：配位-有机溶剂法 银杏叶提取物 银杏酚酸 氯化锌 抗肝癌细胞(HepG2)活性 

分 类 号：S792.95[农业科学—林木遗传育种] TQ460[农业科学—林学]