培养基种类及激素配方对青钱柳悬浮细胞次生代谢物质积累的影响  

Influence of types of basic medium,hormone formulations on accumulation of secondary metabolites in Cyclocarya paliurus suspension cells

作　　者：阮氏钏[1,2,3] 杨万霞[1,2] 方升佐[1,2] 曹燕妮[1,2] 

机构地区：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037 [2]南京林业大学林学院,江苏南京210037 [3]College of Forestry,Vietnam Bac Giang Agriculture-Forestry University

出　　处：《南京林业大学学报（自然科学版）》2015年第2期47-51,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金项目(31270673);江苏省研究生培养创新工程(CXLX12-0540);江苏高校协同创新计划资助项目;江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：以青钱柳无菌苗诱导出的愈伤组织为材料进行悬浮培养,通过控制基本培养基的种类和激素配方,初步筛选出适合于青钱柳次生代谢产物生产的植物悬浮细胞培养体系。结果表明:青钱柳悬浮细胞培养的最佳基本培养基为MS培养基,激素配方为1.0 mg/L NAA+0.5 mg/L KT;有利于青钱柳悬浮细胞的干质量增加、次生代谢产物生产的基本培养基为MS培养基;有利于青钱柳悬浮细胞总黄酮积累的激素配方B1为1.0 mg/L NAA+0.5mg/L KT,有利于青钱柳悬浮细胞多糖积累的激素配方B3为1.0 mg/L IBA+0.5 mg/L TDZ,有利于青钱柳悬浮细胞总三萜积累的激素配方B2为0.5 mg/L 2,4-D+0.3 mg/L NAA。Cyclocarya paliurus callus induced with its aseptic seedling were cultured in suspension culture. The types of basic medium,hormone formulations were studied to select the suitable Cyclocarya paliurus production of secondary metabolites in plant cell suspension culture system. The results were shown as follows： The best medium of Cyclocarya paliurus suspension cell culture medium was MS,hormone formulations was 1. 0 mg/L NAA ＋ 0. 5 mg/L KT. The basic medium was MS which could increase the suspension cell dry weight and the production of secondary metabolites in the medium. B1（1. 0 mg/L NAA＋ 0. 5 mg/L KT）hormone formulations was propitious to flavonoids accumulation in cell suspension. B3（1. 0 mg/L IBA ＋ 0. 5mg/L TDZ） hormone formulations was propitious to polysaccharides accumulation in cell suspension. B2（0. 5 mg/L 2,4-D ＋0. 3 mg/L NAA）hormone formulations was propitious to triterpenoid accumulation in cell suspension. Types of basic medium,hormone formulations on accumulation of secondary metabolites in Cyclocarya paliurus suspension cells were preliminarily selected. The results laid the foundation for the Cyclocarya paliurus suspension cell culture system.

关 键 词：青钱柳 悬浮细胞 总黄酮 总三萜 总多糖 

分 类 号：S792.12[农业科学—林木遗传育种]