马占相思和大叶相思优树组培不定根诱导研究  

Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees

作　　者：胡峰 施琼[2] 黄烈健[2] 

机构地区：[1]广东省农业科学研究院作物研究所,广东广州510640 [2]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《南京林业大学学报（自然科学版）》2015年第2期57-62,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金："十二五"国家科技支撑计划(2012BAD01B0402)

摘　　要：以10年生马占相思和大叶相思优良单株为研究对象,其茎段外植体通过初代培养和增殖培养后获得增殖芽,研究了生长素种类及浓度、基本培养基类型、蔗糖浓度和活性炭浓度对增殖芽生根的影响,分析了不同类型增殖培养基多次继代诱导的增殖芽对生根的影响。结果表明:马占相思的最佳生根培养基为1/2MS+0.5 mg/L IBA+0.5 mg/L NAA+3%蔗糖,大叶相思最佳生根培养基为1/2MS+0.5 mg/L IBA+0.25 mg/L NAA+3%蔗糖,二者的生根率均能达到93%以上。以马占相思和大叶相思增殖芽为生根材料,含低浓度细胞分裂素且添加活性炭的增殖培养基诱导时,两种相思的生根率均能达到90%以上,生根率随着继代次数的增加保持稳定,生根苗生长健壮。Ten years old Acacia mangium and A. auriculiformis elite individual trees were chosen as study materials in this experiment. Proliferated buds were obtained from stem explants being primarily cultured and proliferatively cultured.This study investigated factors related to rooting development of proliferated buds,such as type and concentration of auxins,type of basic medium,concentration of sucrose and active carbon. On the other hand,this experiment also studied the effects of proliferated buds induced by different medium on rooting rate. Results showed that the optimal rooting medium for A. mangium and A. auriculiformis was 1 /2MS ＋ IBA 0. 5 mg/L ＋ NAA 0. 5 mg/L ＋ sucrose 3% and 1 /2MS ＋IBA 0. 5 mg/L ＋ NAA 0. 25 mg/L ＋ sucrose 3%,respectively. The rooting rate of both species could reach above 93%.Rooting rate of proliferated buds,after being cultured on proliferation medium with low concentration of cytokinin and active carbon,could reach above 90%,and remain stable with the increase of subculture time. Rooting seedlings grow strongly.

关 键 词：马占相思 大叶相思 不定根诱导 

分 类 号：S722[农业科学—林木遗传育种]