结核分枝杆菌HspX基因真核表达载体构建  

Construction of Eukaryotic Expression Vector of Mycobacterium Tuberculosis Hsp X Gene

作　　者：髙莉莉 徐苏娟 张继明 王建杰[1] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154002 [2]牡丹江医学院红旗医院检验科,黑龙江牡丹江157011 [3]彩虹医院,陕西咸阳712021

出　　处：《江苏科技信息》2015年第7期41-42,共2页Jiangsu Science and Technology Information

摘　　要：为构建结核分枝杆菌Hsp X基因与增强型绿色荧光蛋白(EGFP)融合基因的真核表达载体,文章采用PCR法扩增Hsp X基因,定向插入到质粒p EGFP-N1的多克隆位点。结果显示,融合基因真核表达载体,经双酶切鉴定、基因测序鉴定克隆片段大小和序列正确。文章成功构建了p EGFP-N1-Hsp X融合基因真核表达载体。In order to construct a recombinant plasmid carrying enhanced green fluorescent protein（EGFP）and Mycobacterium tuberculosis Hsp X gene,Hsp X gene was amplified by PCR and inserted into a multiple cloning site of eukaryotic expression vector p EGFP-N1. The results shows Hsp X eukaryotic expression vector labeled with FLAG tag was successfully constructed as demonstrated by double enzyme digestion and DNA sequencing. The conclusion is that the eukaryotic expression plasmid p EGFP-N1-Hsp X has been constructed successfully.

关 键 词：结核分枝杆菌 HSP X 真核表达 

分 类 号：Q782[生物学—分子生物学]