HPLC-DAD法同时测定消乳增胶囊的三种活性成分  

Determination of Three Active Components in XiaoRuZeng Capsules by HPLC-DAD Method

作　　者：王锐[1,2] 陆铖[1,2] 葛斌[1] 袁继勇[1] 高庆剑 汤浩[1] 

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000 [2]宁夏医科大学药学院

出　　处：《西部中医药》2015年第3期25-28,共4页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省中医药科研立项课题(编号GZK-2010-4);甘肃省省自然科学研究基金计划项目(编号1107RJZA130)

摘　　要：目的：建立HPLC-DAD法同时测定消乳增胶囊中的多种活性成分。方法：采用DAD全波长扫描,色谱柱为Gemini C18（250 mm x4.60 mm,5μm）,流动相为乙腈,甲醇为10：90（A）-0.5%冰醋酸水溶液（B）梯度洗脱,流速为0.8 m L/min;检测波长为280 nm。结果：甘草苷线性范围为1.25-40μg/m L,平均回收率为98.51%,RSD为0.81%;橙皮苷线性范围为2.5-80μg/m L,平均回收率为98.10%,RSD为1.32%;欧前胡素线性范围为5-160μg/m L,平均回收率为98.92%,RSD为0.76%。结论：该HPLC-DAD方法能有效测定消乳增胶囊中甘草苷、橙皮苷及欧前胡素的主要活性成分含量,且快速、准确。Objective： To determine the multi-active components in Xiao Ru Zeng capsules by a HPLC-DAD method. Methods： With DAD full wavelength scan, the determined conditions were follows： chromatographic column： Gemini C18（250 mm× 4.60 mm）; mobile phase： acetonitrite/methanol（10：90）（A） and 0.5% acetic acid（B）with gradient elution; flow rate： 0.8 m L/min; detection wavelength： 280 nm. Results： The results revealed that the linear range of liquiritin was 1.25-40 μg/m L, the average recovery 98.51% as well as RSD 0.81%, the linear range of hesperidin was 2.5~80 μg/m L, the average recovery 98.10% as well as RSD 1.32% and the linear range of imperatorin was 5~160 μg/m, the average recovery 98.92% as well as RSD 0.76%. Conclusion： The HPLC-DAD method is accurate and easy in determining the main components of Xiao Ru Zeng capsules like hesperidin, liquiritin and imperatorin.

关 键 词：HPLC-DAD “消乳增”胶囊 橙皮苷 甘草苷 欧前胡素 

分 类 号：R285[医药卫生—中药学]