胰岛素抵抗OLETF大鼠血清IL-18水平变化及替米沙坦的干预研究  

Change of interleukin-18 in OLETF rats with insulin resistance and effects of telmisartan

作　　者：王伟占 孙立亭[1] 赵志强[3] 田凤石[4] 

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津医科大学第四中心临床学院心内科,天津300140 [3]天津医科大学第二医院心内科,天津300211 [4]天津市第四中心医院心内科,天津300140

出　　处：《天津医科大学学报》2015年第2期101-104,共4页Journal of Tianjin Medical University

摘　　要：目的:测定胰岛素抵抗(IR)OLETF大鼠血清白介素-18(IL-18)的表达水平,研究血管紧张素Ⅱ1型受体拮抗剂(AT1R)替米沙坦对IL-18水平的影响及对IR的干预作用,探讨其改善IR的可能机制。方法:OLETF大鼠24只,高脂喂养20周诱导建立IR模型,将成功造模的24只IR大鼠随机分为2组,模型(M)组12只继续给予高脂饲料,替米沙坦(T)组12只给予替米沙坦[5 mg/(kg·d)]加高脂饲料,LETO大鼠12只为对照(NC)组,给予标准饲料。干预20周后测定两组大鼠的体质量(W)、血清胰岛素(FINS)、空腹血糖(FBG)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)及IL-18水平,并计算胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(ISI)。结果:M组大鼠与NC组大鼠相比,W、FBG、TG、LDL-C及IL-18水平明显增高(P<0.05或P<0.01);T组与M组相比,FBG、TG、LDL-C、IL-18水平明显降低(P<0.05或P<0.01)。多元线性回归分析提示IL-18是HOMA-IR的独立影响因素。结论:IL-18可能通过促进炎症参与了IR,而替米沙坦可能通过调节糖脂代谢、抑制前炎性因子IL-18的表达改善IR。Objective： To investigate change of interleukin-18（IL-18） in OLETF rats with insulin resistance（IR）, and to study the effect of angiotensin II type 1 receptor antagonists（AT1R） telmisartan on IR and the expression of IL-18, and the possible mechanisms of telmisartan improving IR. Methods： Twenty-four OLETF rats were fed with high-fat diet for 20 weeks to establish the IR model. Twenty-four OLETF rats with IR were randomly assigned into two groups： the model group（group M）, in which the rats were fed with high-fat diet; the telmisartan group（group T）, in which the rats were fed with high-fat diet and telmisartan[5 mg/（kg·d）]. Twelve LETO rats fed with normal diet served as normal control group（group NC）. After 20 weeks of drug intervention, the weight（W）,plasma levels of fasting insulin（FINS）, fasting blood glucose（FBG）, triglycerides（TG）, high-density lipoprotein cholesterol（HDL-C）, low-density lipoprotein cholesterol（LDL-C） and IL-18 were detected in two groups. And HOMA-IR and ISI were measured. Results： Compared with group NC, the W,FBG,TG,LDL-C and IL-18 were significantly increased in group M（P〈0.05 或 P〈0.01）; compared with group M, the FBG,TG,LDL-C and IL-18 were significantly decreased in group T（P〈0.05 或 P〈0.01）. The multiple linear regression analysis showed that the serum IL-18 was an independent determinant of HOMA-IR. Conclusion： The IL-18 is an independent factor for IR, and telmisartan can improve IR by regulating blood glucose and serum lipid as well as inhibiting the expression of IL-18.

关 键 词：胰岛素抵抗 替米沙坦 OLETF大鼠 白介素-18 

分 类 号：R589[医药卫生—内分泌]