重组人粒细胞巨噬细胞集落刺激因子凝胶对深II度烧伤创面愈合的影响及其机理分析  

Effects of recombinant human granulocyte-macrophage colony-stimulating factor on debridement of deep partialthickness burn

作　　者：刘继松[1] 赵经纬[1] 章祥洲[1] 杜娟[1] 方勇[2] 

机构地区：[1]安徽省蚌埠市第三人民医院烧伤整形科,233000 [2]上海交通大学第三附属人民医院烧伤整形科,上海201999

出　　处：《淮海医药》2015年第3期219-221,共3页Journal of Huaihai Medicine

摘　　要：目的验证重组人粒细胞巨噬细胞集落刺激因子（rh GM-CSF）对深II度烧伤溶痂和愈合的效果并分析其可能的机理。方法 70只SD大鼠制成背部深II度烧伤创面后随机分为实验组和对照组,分别于制创后1、3、5、7、10、14和21d观察2组动物创面情况并摄像,记录创面愈合时间;用图像分析软件计算不同时相点的溶痂率;ELISA法测定创面弹力蛋白酶（NE）和基质金属蛋白酶（MMP-1、MMP-9）的蛋白表达。结果实验组创面溶痂时间为（10.73±2.47）d,较对照组（14.26±2.65）d显著缩短（P〈0.01）;实验组和对照组创面愈合时间分别为（16.21±1.27）d和（18.05±1.36）d,差异有非常显著性（P〈0.01）;2组动物创面伤后3-7 d,NE、MMP-1和MMP-9水平实验组明显高于对照组（P〈0.01）。结论外源性rh GM-CSF的应用可提高创面局部NE、MMP-1、MMP-9的蛋白表达,促进创面溶痂,从而促进深II度烧伤创面愈合。Objective To determine the effect of recombinant human granulocyte-macrophage colony-stimulating factor（ rhGM-CSF） on debridement of eschar in deep partial thickness scalding wound and the mechanism for debridement of rh GMCSF. Methods A total of 70 SD rats were scalded on the back. A deep partial-thickness burn was created in all the rats. The scalded rats were randomly divided into a control group（ C） and an experimental group（ E）. Observations of the wounds were conducted by photograph at various time-points. In addition,the removal time of wound eschar in all animals was recorded. The percentages of removal area in the wounds were calculated by image analysis software in various time-points. ELISA was used to detect the expression of NE,MMP-1,and MMP-9. Results The average removal-time of eschar in the experimental group was10. 73 ± 2. 47 d,which was much shorter than that in the control group＇s 14. 26 ± 2. 65d（ P〈0. 01）. The time of wound healing in the experimental group was 16. 21 ± 1. 27 d,which was significantly shorter than that in the control group＇s 18. 05 ± 1.36d（ P〈0. 01）. The levels of NE,MMP-1 and MMP-9 in the experimental group were significantly higher than that in the control group from day 3 to 7 after injury（ P〈0. 01）. Conclusion Rh GM-CSF gel may promote debridement and wound healing in deep partial thickness burn,the mechanism of which may be associated with upregulation of expression of the enzyme activity.

关 键 词：烧伤 深II度 创面愈合 溶痂 粒细胞巨噬细胞集落刺激因子 

分 类 号：R644[医药卫生—外科学]