莲草直胸跳甲Hsp70蛋白的生物信息学分析  

Bioinformatic Analysis of Agasicles hygrophila Hsp70

作　　者：贾栋[1] 赵龙龙[1] 王森[1] 付淑慧[2] 郭艳琼[1] 马瑞燕[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学生命科学院,山西太谷030801

出　　处：《山西农业科学》2015年第4期397-400,共4页Journal of Shanxi Agricultural Sciences

基　　金：教育部博士点基金项目(20111403110004);山西农业大学中青年拔尖创新人才支持计划项目(BJRC201201)

摘　　要：根据莲草直胸跳甲Hsp70基因的EST序列,利用电子克隆的方法获得莲草直胸跳甲Hsp70基因,通过生物信息学方法对该基因编码蛋白进行了保守结构域、疏水/亲水性、亚细胞定位、信号肽等理化性质和分子特性分析。结果表明,莲草直胸跳甲Hsp70基因全长2 216 bp,完整读码框1 899 bp,编码632个氨基酸残基,分子量为69.45 k Da,等电点为6.00;编码蛋白含有Hsp70的完整保守结构域和典型签名序列;具有的典型细胞质基序和亚细胞定位分别表明Hsp70位于细胞质中。研究结果可为深入研究该基因的生物学功能奠定基础。Hsp70 was cloned based on its EST sequences from Agasicles hygrophila. Physicochemical properties and molecular features of Hsp 70 were predicted by bioinformatics approaches including conserved domain, hydrophobic or hydrophilic, subcellular lo- calization, signal peptide. The results showed that the full-length Hsp 70 was 2 216 bp, and it contained a complete ORF which encoded 632 amino acids, and a molecular weight of 69.45 kDa, isoe｜ectric point of 6.00; Hsp70 contained complete conserved domain and typical sequence; its typical cytoplasmic motif and subcellular localization had indicated that Hsp70 located in the cytoplasm. The results will provide basis for the in-depth study of biological function of Hsp70.

关 键 词：莲草直胸跳甲 HSP70 电子克隆 生物信息学分析 

分 类 号：S451[农业科学—植物保护]