槐耳颗粒在体外对结肠癌SW480细胞侵袭转移的影响  

Effect of Huaier Granules on Invasion and Metastasis of Colorectal Cancer SW480 Cells in vitro

作　　者：姜建武 李小兰[1] 冷彦[1] 陶德定[1] 胡俊波[1] 龚建平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胃肠外科/分子医学中心,武汉430030

出　　处：《医药导报》2015年第4期455-458,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81101943;81171927;81372324;81201638)

摘　　要：目的观察槐耳颗粒在体外对结肠癌SW480细胞侵袭转移的影响,并初步探讨其机制。方法 Sub G1法确定槐耳颗粒促进结肠癌SW480细胞凋亡的最佳作用浓度和时间,通过划痕愈合实验和Transwell侵袭实验观察槐耳颗粒对结肠癌SW480细胞侵袭转移的影响。Western blot法和Real-Time PCR(荧光定量PCR)法检测E-cadherin、twist、snail、vimentin在蛋白和mRNA水平的变化。结果槐耳颗粒3.0 g·L-1处理SW480细胞36 h后凋亡最明显。槐耳颗粒3.0 g·L-1处理SW480细胞36 h后,划痕愈合实验相对迁移率为(31.36±2.39)%,而对照组36 h后相对迁移率则为(61.11±1.09)%(P<0.01)。Transwell侵袭实验证实槐耳颗粒3.0 g·L-1处理SW480细胞36 h后每个视野穿透细胞数为(129±12)个,而对照组为(354±20)个(P<0.01)。槐耳颗粒3.0 g·L-1处理SW480细胞36 h后E-cadherin在蛋白和mRNA水平表达均升高,而twist、snail和vimentin表达均降低。结论槐耳颗粒在体外能够通过抑制上皮-间质转化有效抑制结肠癌SW480细胞的侵袭转移。Objective To investigate the effects of huaier granules on invasion and metastasis of colorectal cancer SW480 cells in vitro, and explore the basic mechanism. Methods The appropriate concentration and duration of huaier granules promoting SW480 cell apoptosis were determined by SubG1 method. Wound healing assay and transwell assay were used to observe the effect of huaier granules on SW480 cell invasion and metastasis. The changes of E-cadherin, twist, snail and vimentin at protein and mRNA levels were examined by Western blotting and Real-Time PCR. Results After treatment with huaier granules at 3. 0 g·L-1 for 36 h, apoptosis of SW480 cells was most significant, and wound healing assay revealed that relative mobility was （31. 36±2. 39）%, compared with （61. 11±1. 09）% in control group （P〈0. 01）. Number of invaded cells per field of view was （129±12） in treatment group and （354±20） in control group （P〈0. 01）. After treatment with huaier granules at 3. 0 g·L-1 for 36 h, protein and mRNA levels of E-cadherin were increased, while those of twist, snail and vimentin were decreased. Conclusion Huaier granules can inhibit invasion and metastasis of colorectal cancer SW480 cells in vitro through effectively depressing epithelial-mesenchymal transition.

关 键 词：槐耳颗粒 癌 结直肠 细胞 SW480 上皮-间质转化 侵袭 转移 

分 类 号：R286[医药卫生—中药学] R285.5[医药卫生—中医学]