中空纤维液相微萃取结合高效液相色谱法测定比索洛尔血浆蛋白结合率  

Determination of Plasma Protein Binding Rate of Bisoprolol in Human Plasma by Hollow Fiber Liquid-phase Microextraction Combined with HPLC

作　　者：刘亚非[1,2] 马海英[1,2] 宫雪菲[1,2] 王宝华[1,2] 

机构地区：[1]中国医科大学附属第四医院药学部,沈阳110032 [2]中国医科大学药学院,沈阳110032

出　　处：《医药导报》2015年第4期529-533,共5页Herald of Medicine

基　　金：辽宁省科学技术计划项目(2011225020)

摘　　要：目的将中空纤维液相微萃取技术与高效液相色谱法结合,建立测定比索洛尔血浆蛋白结合率的新方法。方法优化比索洛尔血浆样本的液相微萃取条件;利用高效液相色谱法测定比索洛尔在接受相中的浓度,色谱条件为水-甲醇-乙腈-0.1%磷酸(50∶34∶6∶10),检测波长Ex:232 nm,Em:300 nm,分别代入当日标准曲线计算出比索洛尔血浆游离药物浓度及总药物浓度,进而计算比索洛尔血浆蛋白结合率。结果在低、中、高3种浓度下测得比索洛尔的血浆蛋白结合率分别为31.2%,32.0%,31.8%。结论所建立的方法简便快速,重复性好,适用于药物游离浓度及血浆蛋白结合率的测定。比索洛尔与人血浆蛋白有中等程度结合,且不存在浓度依赖性。Objective To develop a method for determination of the plasma protein binding rate of bisoprolol in human plasma by high performance liquid chromatography （HPLC） combined with hollow fiber-based liquid-phase microextraction （ HF- LPME）. Methods Method of liquid phase microextraetion was optimized. The concentration of bisoprolol in the reconstitute solution was analyzed by HPLC. The mobile phase consisted of water-methanol-acetonitrile-0.1% phosphoric acid （50 ： 34 ： 6 ： 10）. The excitation wavelength was 232 nm and emission wavelength was 300 nm. Through the linear regression equations, the total and free concentrations were obtained, and then the protein binding rate was calculated. Results At low, middle, and high concentration, the protein binding rate of bisoprolol was 31.2%, 32.0% and 31.8%, respectively. Conclusion The proposed method is proven to be simple, fast and reproducible, and is feasible for the determination of plasma protein binding rate of bisoprolol. Bisoprolol moderately binds with plasma protein independent of concentration.

关 键 词：比索洛尔 微萃取 液相 中空纤维 色谱法 高效液相 结合率 血浆蛋白 

分 类 号：R972[医药卫生—药品] R969.1[医药卫生—药学]