血液1-(3-三氟甲基苯基)哌嗪和1-(3-氯苯基)哌嗪检验  

The detection of mC PP、TFMPP in blood samples by gas chromatographic mass spectrum and gas chromatography with nitrogen phosphorus detection

作　　者：常靖[1] 郝红霞[2] 李红旭[2] 王芳琳[1] 侯小平[1] 王瑞花[1] 

机构地区：[1]公安部物证鉴定中心,北京100038 [2]中国政法大学证据科学研究院,北京100040

出　　处：《中国法医学杂志》2015年第2期121-124,共4页Chinese Journal of Forensic Medicine

基　　金："十二五"国家科技支撑计划课题(2012BAK02B02);中央公益性科研院所基本科研业务专项资金项目(2012JB005)

摘　　要：目的建立血液中1-（3-三氟甲基苯基）哌嗪（TFMPP）、1-（3-氯苯基）哌嗪（m CPP）的检测方法,并用于实际案件检验。方法 Zn SO4沉淀蛋白后,在p H11条件下,用氯仿/异丙醇（4/1,v/v）提取,采用GC/MS与GC/NPD分析。结果 TFMPP、m CPP回收率均在90%以上,在0.25~20μg/m L浓度范围内线性关系良好,r2为0.999 9,最低检测限为0.135μg/m L。结论本方法回收率高,操作简便,可用于血液中TFMPP、m CPP的提取检测。Objective An GC /MS and GC /NPD method was developed for trifluoromethylphenylpiperazine（ TFMPP） and benzylpiperazine（ m CPP） in human blood. Methods Using chloroform / isopropanol（ 4 /1,V/V） extracted the sample under the condition of alkaline p H11. Evaporated under nitrogen to dry and reconstitute with 100μL methanol prior to GC-MS and GC / NPD analysis. Results The recoveries obtained with this method for TFMPP and m CPP were higher than 90%. The linear range for TFMPP and m CPP in blood was evaluated for the concentrations： 0. 25- 20μg / m L with correlation coefficients 0. 999 9.The limits of detection,based on a signal to noise ratio（ S / N） of 3 ∶ 1,were 0. 135μg / m L. Conclusion The method developed had good recovery,matric effect was small and interference was well depurated,it could be applied for separating and extracting TFMPP and m CPP in blood samples with small quantity.

关 键 词：法医毒物分析 GC-MS GC/NPD 1-(3-三氟甲基苯基)哌嗪 1-(3-氯苯基)哌嗪 血液 

分 类 号：D919[医药卫生—法医学]