液相色谱-质谱联用法测定生物样品中阿立哌唑与氯氮平  

Determination of aripiprazole and clozapine in biological samples by liquid chromatography-mass spectrometry

作　　者：张美玲[1] 张园[1] 王学宝[1] 张振南[1] 马建设[1] 林丹[1] 王贤亲[1] 

机构地区：[1]温州医科大学司法鉴定中心法医毒物实验室,浙江温州325035

出　　处：《中国法医学杂志》2015年第2期141-143,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立一种灵敏、简单测定生物样品中阿立哌唑与氯氮平的液相色谱-质谱联用法。方法样品处理采用液-液乙酸乙酯萃取方法。色谱柱为Zorbax SB-C18（2.1 mm×150 mm,5μm）,流动相为乙腈与0.1%甲酸,梯度洗脱,流速为0.4 ml/min,柱温30℃。采用ESI离子源,MRM离子方式监测。结果阿立哌唑与氯氮平在50~2 000ng/m L浓度范围内线性良好（r〉0.993）;回收率均在87%~112%范围内,日内、日间RSD均小于12%。结论本方法可简单、高效地检测血液与尿液中阿立哌唑与氯氮平浓度。Objective To develop a sensitive and simple liquid chromatography-mass spectrometry method for determination of aripiprazole and clozapine in biological samples. Methods Aripiprazole and clozapine were extracted from rat plasma by liquid-liquid extraction with ethyl acetate,and then analyzed on a Zorbax SB-C18（ 2. 1mm × 150 mm,5μm） column. The mobile phase consisted of acetonitrile-0. 1%formic acid with gradient elution,at a flow rate of 0. 4 ml / min,at 30℃. LC-MS was performed in ESI source with MRM mode for quantification. Results The linear range of the concentration were 50- 2 000 ng / m L for aripiprazole and clozapine（ r 〉0. 993）. The relative recoveries of aripiprazole and clozapine were in the range of 87%- 112%. The RSDs of intra-days and inter-day were both less than 12%. Conclusion The method is simple,quick,sensitive and could be used for determination of aripiprazole and clozapine in biological samples.

关 键 词：法医毒物分析 液-质联用法 阿立哌唑 氯氮平 生物样品 

分 类 号：D919[医药卫生—法医学]