电磁脉冲诱导大鼠左心室肌组织基因表达谱的变化  

Change of chart genes expression in left ventricle myocardium of rats induced by electromagnetic pulses irradiation

作　　者：李宝丰[1] 任东青[2] 郭国祯[2] 陈蓓[3] 章莹[1] 夏虹[1] 

机构地区：[1]广州军区广州总医院骨科,广东广州510010 [2]第四军医大学放射医学教研室,陕西西安710032 [3]广州军区广州总医院肿瘤科,广东广州510010

出　　处：《东南大学学报（医学版）》2015年第2期171-176,共6页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81172636;81000819)

摘　　要：目的:用基因芯片技术研究电磁脉冲(electromagnetic pulse,EMP)辐照对大鼠左心室肌组织的生物效应。方法:10只雄性同窝SD大鼠随机分为辐照组与正常对照组2组,每组5只。在锥形平板GTEM小室内对辐照组大鼠进行EMP辐照,EMP辐照参数为:场强200 k V·m-1,脉冲前沿3.5 ns,脉宽14 ns,重复频率1 Hz;正常对照组进行假辐照。辐照后18 h取大鼠左心室肌,提取RNA,经反转录后用Cy3、Cy5荧光标记,获得两组动物来源c DNA探针,c DNA探针与基因表达谱芯片进行杂交,结果由扫描仪扫描并用软件进行分析统计。结果:筛选出差异表达基因108条,表达增强的有51条,其中功能已知或部分已知的11条,占22%;表达减弱的有57条,其中功能已知或部分已知的15条,占26%。差异表达基因主要涉及代谢、神经递质、肌肉收缩、凋亡、应激等方面。其中蛋白激酶C、热休克蛋白70、辅酶Q10、亚甲基四氢叶酸脱氢酶、5-羟色胺受体2C、锌指蛋白10、干扰素γ、雄激素调节蛋白、雌激素调节蛋白等可能在EMP对大鼠心脏生物学效应方面起重要作用。结论:EMP辐照可诱导大鼠左心室肌组织多个基因特异性表达。Objective： To explore the bio-effects of electromagnetic pulse（ EMP） on left ventricle myocardium of rats by the means of gene chip. Methods： 10 male SD rats were randomly divided into two groups with 5 for each group. One group was subjected to EMP fields（ 200 k V·m^-1,3. 5 ns duration,14 ns width,1 Hz） and the other as control. 18 hours after the irradiation,the rats were sacrificed and their left ventricle myocardium were eviscerated. The fluorescent c DNA probes labeled with Cy3 and Cy5 were prepared from RNA extracted from the ventricle myocardium of the two groups. Probes of the two groups were then hybridized against c DNA gene chip,the fluorescent signals were scanned with a scanner and the results were analyzed by computer. Results： Compared with the control,108 genes in gene expression profile were altered. The expression levels of 51 genes were upregulated distinctly while 57 genes were down-regulated significantly involved in metabolism,neurotransmitter,muscle contraction,apoptosis,stress response and so on. Among the 108 genes,PKC,HSP70,ubiquinone oxidoreductase,methylene tetrahydrofolate dehydrogenase,5-hydroxytryptamine receptor 2C,zinc finger protein10,interferon gamma,androgen regulated 20 k Da protein,estrogen receptor associated protein and so on might have important roles on the cardiac biological effects by EMP. Conclusion： EMP exposure in this study can induce specific expressions of some genes in left ventricle myocardium of rats.

关 键 词：电磁脉冲 大鼠 心肌 基因芯片 表达 

分 类 号：R82[医药卫生—临床医学]