盐酸氯苯胍灌服后在兔尿液和粪便中的排泄规律  

Studies on the excretion of robenidine hydrochloride in rabbit urine and feces

作　　者：田苗苗[1] 郭春娜[1] 怀彬彬 郜进[1] 黄显会[1] 

机构地区：[1]华南农业大学国家兽药残留基准实验室,广东广州510642

出　　处：《华南农业大学学报》2015年第3期8-12,共5页Journal of South China Agricultural University

基　　金：农业行业标准制修订项目(201391194)

摘　　要：【目的】建立氯苯胍在兔尿液和粪便中的高效液相色谱检测方法,以测定氯苯胍在兔排泄物中的排泄量.【方法】12只健康新西兰大白兔灌服盐酸氯苯胍15mg·kg^-1后,于不同时间间隔收集其所有粪便和尿液,粪便称质量、尿液定容后冻存.称取0.2g粪便,用体积比为2∶1的三氯甲烷-甲醇混合液提取,氮气吹干;量取1mL兔尿,用体积分数为0.2%乙酸酸化的乙酸乙酯提取,氮气吹干,残渣均用乙腈-水（体积比30∶70）溶液复溶,CNWHLB柱净化,浓缩后用甲醇定容,上机检测.【结果和结论】氯苯胍在兔尿中的检测限与定量限分别为0.005和0.01μg·mL^-1,通过尿液排泄的氯苯胍占给药总量的比例为0.56%;在兔粪便中的检测限与定量限分别为0.01和0.05μg·g^-1,通过粪便排泄的氯苯胍占给药总量的比例为87.17%.氯苯胍在兔尿和粪便中排出高峰期分别在给药后0～6和6～12h,占给药后累积排泄总量的比例分别为28.37%和38.46%.结果显示,该检测方法满足测定氯苯胍在兔尿和粪便中排泄量的要求,内服盐酸氯苯胍后大部分氯苯胍随尿液和粪便排出体外.【Objective】In order to investigate the excretion quantitation of robenidine in rabbit urine and feces,a high performance liquid chromatography( HPLC) method was developed to detect the concentrations of robenidine hydrochloride. 【Method】Twelve healthy rabbits were administered robenidine hydrochloride orally with a dose of 15 mg·kg- 1. After administration,all of their urine and feces were collected at different time intervals and mass were achieved. Feces 0. 2 g was extracted using chloroform-methanol [V( chloroform) ∶ V( methanol) = 2∶ 1]; urine 1 m L was extracted with φ = 0. 2% acidified ethyl acetate,and the organic phase was separated and evaporated. The residue was redissolved with acetonitrilewater [V( acetonitrile) ∶ V( water) = 30 ∶ 70] solution,and cleaned up by solid-phase extraction using CNW HLB after being evaporated to dryness; the residue was redissolved in methanol,and then analyzed by HPLC. 【Result and conclusion】The results showed that the limit of detection( LOD) and the limit of quantitation( LOQ) of robenidine in urine were 0. 005 and 0. 01 μg·m L- 1respectively,with the proportion of the total administered robenidine being 0. 56%; the LOD and the LOQ of robenidine in feces were 0. 01 and 0. 05 μg·g- 1respectively,with the proportion of the total administered robenidine being87. 17%. The excretion peak time of robenidine in urine and feces was 0- 6 and 6- 12 h respectively,and the total amount of accumulated excretion was 28. 37% and 38. 46% respectively after administration. The results indicate that this method can be used to detect the concentration of robenidine in rabbiturine and feces,and most of the administered drug is excreted in the original form of urine and feces.

关 键 词：兔 排泄物 氯苯胍 高效液相色谱法 残留检测 

分 类 号：S859.795[农业科学—临床兽医学]