洋葱伯克霍尔德溶磷菌的筛选和溶磷培养条件优化  

Screening of phosphorus-solubilizing strain Burkholderia cenocepacia and optimizing of phosphate-dissolving culture condition

作　　者：刘云华[1] 吴毅歆[2,3] 杨绍聪 何鹏飞[1] 何月秋[1,2] 

机构地区：[1]云南农业大学农业生物多样性应用技术国家工程研究中心,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]微生物菌种筛选与应用国家地方联合工程研究中心,云南昆明650217 [4]玉溪市农业科学院,云南玉溪653100

出　　处：《华南农业大学学报》2015年第3期78-82,共5页Journal of South China Agricultural University

基　　金：科技部国际科技合作项目(2009DFA32360);昆明市科技局项目(09H130301)

摘　　要：【目的】从磷矿区植物根围土中,筛选出具有较高溶磷能力的菌株及优化溶磷培养条件.【方法】通过稀释凃板法分离、筛选菌株,并进行全细胞脂肪酸分析和16S r DNA测序鉴定;正交试验优化溶磷培养条件.【结果和结论】筛选出22株具有溶磷能力的菌株,其中菌株YN2014102的溶磷能力最强,在10 g·L-1的磷矿粉培养基中能释放244.75 mg·L-1水溶性磷.经全细胞脂肪酸分析和16S r DNA测序,该菌株被鉴定为洋葱伯克霍尔德菌.正交试验的结果表明,在质量浓度10 g·L-1的磷矿粉、28℃、摇床转速为180 r·min-1的条件下培养5 d,溶磷效果最好,达277.08 mg·L-1.【Objective】To isolate and screen phosphorus-solubilizing strains from the rhizospheric soil in phosphate ore zone and to optimize its phosphorus-solubilizing condition. 【Method】Dilution and plating were applied to screen and purify phosphorus-solubilizing strains. The whole-cell fatty acid was analyzed by gas chromatography and 16 S r DNA sequence was identified. The orthogonal design test was used in optimizing phosphorus-solubilizing condition. 【Result and conclusion 】Total 22 phosphorus-solubilizing strains were obtained by the traditional methodologies. YN2014102 strain showed a strong ability to dissolve phosphate,which could get 244. 75 mg·L- 1available phosphorus from the 10 g·L- 1phosphate rock medium.YN2014102 strain was identified as Burkholderia cenocepacia based on its gas chromatography analysis of whole-cell fatty acid and 16 S r DNA sequencing. When the bacterium was cultured in the 10 g·L- 1phosphate rock powder medium at 28 ℃ in a shaker with 180 r·min- 1for 5 days,the dissolve effect of solubilizing phosphorus was the best,which can obtain 277. 08 mg·L- 1of available phosphorus in the medium.

关 键 词：溶磷细菌 磷矿粉 气相色谱分析 全细胞脂肪酸 16S rDNA 

分 类 号：S154.3[农业科学—土壤学]