太空飞行对大肠杆菌生物学性状影响的研究  

Effects of Space Flight on Biological Characteristics of Escherichia Coli

作　　者：张学林[1] 谢琼[2] 刘长庭[1] 

机构地区：[1]中国人民解放军总医院南楼呼吸科,北京100853 [2]中国航天员训练中心医监医保研究室,北京100094

出　　处：《载人航天》2015年第2期200-204,共5页Manned Spaceflight

基　　金：国家重点基础研究发展计划资助(973计划)(2014CB744400);全军医学科研"十二五"课题重点项目(BWS12J046);科技部重大专项项目(空间新药重大创制)(2015ZX09J15102-003);北京市科技新星项目(z121107002512128)

摘　　要：为探讨空间环境对大肠杆菌生物学性状的影响,通过神舟十号飞船将大肠杆菌送至外太空(太空组),地面设立同步温度对照组,返回后进行菌落计数,观察太空飞行对细菌繁殖的影响;进行亚胺培南敏感性检测,了解耐药性变化;进行生长曲线测定,了解飞行后的生长速率。结果发现太空组细菌量为7.9×1010cfu/m L,对照组为28.5×1010cfu/m L;太空组平均抑菌圈直径为15.57±1.2 mm;对照组为13.4±1.15 mm;两组菌的生长曲线没有明显差异。研究表明大肠杆菌在太空飞行环境中生长受抑制,且对亚胺培南耐药性降低。To manifest the effect of space flight on biological characteristic in Escherichia coli,the strain was carried to space by Shenzhou-10 spaceship. The colony forming units( CFU) in both the space flight and ground cultured E. coli were assessed by plate clone count respectively to calculate the survival rate of space flight E. coli sample. Antibiotics sensitivity for space flight and ground cultured E. coli was analyzed to compare the sensitivity to imipenem. Then the growth curves of the two strains were measured. The results show that,after space flight,the CFU of E. coli was 7. 9 × 1010 cfu / m L,while it was 28. 5 × 1010 cfu / m L in ground cultured E. coli. The average inhibition zone of imipenem for ground cultured E. coli was 13. 4 ± 1. 15 mm in diameter,while that of the space flight gruop was 15. 57 ± 1. 2 mm in diameter. However,the growth curves of the two strains had no significant difference. All data revealed that the growth of E. coli was inhibited in space flight environment,and it could become less resistant to imipenem after space flight.

关 键 词：大肠杆菌 太空飞行 亚胺培南 耐药 生长 

分 类 号：R856[医药卫生—航空、航天与航海医学]