Structural insights into Rhino-mediated germline piRl~ cluster formation  

作　　者：Bowen Yu Madeline Cassani Min Wang Mofang Liu Jinbiao Ma Guohong Li Zhao Zhang Ying Huang 

机构地区：[1]State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Chinese Academy of Sciences, Shanghai 200031, China [2]Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA [3]National Laboratory of Biomacromolecules, Chinese Academy of Sciences, Beijing 100101, China [4]State Key Laboratory of Genetic Engi- neering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200438, China [5]University of Chinese Academy of Sciences, Beijing 100049. China [6]Collaborative Innovation Center of Genetics and Development, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Scienc- es, Chinese Academy of Sciences, Shanghai 200031, China

出　　处：《Cell Research》2015年第4期525-528,共4页细胞研究（英文版）

基　　金：We thank Prof William E Theurkauf at University of Massachusetts Medical School for providing the Rhino plasmids. This work was supported by grants from the Ministry of Science and Technology of China （2011CB966304, 2012CB910502）, the National Natural Science Foundation of China （31270774）, and the Strategic Priority Research Program （XDB08010202）. We thank the staff at BL17U of Shanghai Synchrotron Radiation Facility （SSRF） for their help with data collection.

摘　　要：Dear Editor, The piRNA pathway silences transposons in animal gonads. Animals lacking piRNA pathway components often show transposon activation in the germline and compromised fertility [1]. In Drosophila germ cells, piRNA precursors are transcribed from heterochromatic piRNA cluster regions, and then transported into nuage/ mitochondria to be processed into mature piRNAs that are loaded into PIWI clade Argonautes [1]. Guided by piRNAs, PIWI proteins silence transposons at either transcriptional or post-transcriptional level [2]. Dro- sophila ovaries contain two types of piRNA clusters, which are often coated with trimethylated lysine 9 of H3 （H3K9me3）.

关 键 词：生殖细胞 转录后水平 RNA基因 介导 犀牛 结构 集群 基因沉默 

分 类 号：Q949.718.2[生物学—植物学] S188[农业科学—农业基础科学]