Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins  

作　　者：Ning Xuan Xia Guo Hong-Yan Xie Qi-Nian Lou Xing-Bo Lu Guo-Xia Liu Jean-Francois Picimbon 

机构地区：[1]Biotechnology Research Center, Shandong Provincial Key Laboratory for Genetic Improvement Cultivation, Ecology and Physiology of Crops, Shandong Academy of Agricultural Sciences, Jinan [2]Shandong Silkworm Institute, Shandong Academy of Agricultural Sciences,Yantai [3]plant Protection Institute, Shandong Academy of Agricultural Sciences, Jinan, China

出　　处：《Insect Science》2015年第2期203-219,共17页昆虫科学（英文版）

摘　　要：We analyzed 20 chemosensory protein （CSP） genes of the silkworm Bombyx mori. We found a high number of retrotransposons inserted in introns. We then analyzed expression of the 20 BrnorCSP genes across tissues using quantitative real-time polymerase chain reaction （PCR）. Relatively low expression levels of BmorCSPs were found in the gut and fat body tissues. We thus tested the effects of endectocyte insecticide abamectin （B 1 a and Blb avermectins） on BmorCSP gene expression. Quantitative real-time PCR experi- ments showed that a single brief exposure to insecticide abamectin increased dramatically CSP expression not only in the antennae but in most tissues, including gut and fat body. Furthermore, our study showed coordinate expression of CSPs and metabolic cytochrome P450 enzymes in a tissue-dependent manner in response to the insecticide. The function of CSPs remains unknown. Based on our results, we suggest a role in detecting xenobiotics that are then detoxified by cytochrome P450 anti-xenobiotic enzymes.We analyzed 20 chemosensory protein （CSP） genes of the silkworm Bombyx mori. We found a high number of retrotransposons inserted in introns. We then analyzed expression of the 20 BrnorCSP genes across tissues using quantitative real-time polymerase chain reaction （PCR）. Relatively low expression levels of BmorCSPs were found in the gut and fat body tissues. We thus tested the effects of endectocyte insecticide abamectin （B 1 a and Blb avermectins） on BmorCSP gene expression. Quantitative real-time PCR experi- ments showed that a single brief exposure to insecticide abamectin increased dramatically CSP expression not only in the antennae but in most tissues, including gut and fat body. Furthermore, our study showed coordinate expression of CSPs and metabolic cytochrome P450 enzymes in a tissue-dependent manner in response to the insecticide. The function of CSPs remains unknown. Based on our results, we suggest a role in detecting xenobiotics that are then detoxified by cytochrome P450 anti-xenobiotic enzymes.

关 键 词：ABAMECTIN Bombyx mori chemosensory protein cytochrome P450 oxidase INSECTICIDE MOTH 

分 类 号：S859.795[农业科学—临床兽医学] TF777.7[农业科学—兽医学]