射频电流对成纤维细胞增殖及胶原蛋白表达的影响  

Effect of Radiofrequency Current on Cel ular Proliferation and Col agen Secretion in Human Dermal Fibroblast

作　　者：蔡宏[1] 王毅侠[1] 董宁[2] 孙平[1] 刘玮[1] 

机构地区：[1]解放军空军总医院皮肤科,北京市100141 [2]解放军总医院烧创伤研究所

出　　处：《中国激光医学杂志》2015年第1期32-35,57,共5页Chinese Journal of Laser Medicine & Surgery

摘　　要：目的射频除皱技术在临床上的运用已经相当广泛,并取得了很好的疗效。但缺乏基础研究,以致对射频电流刺激成纤维细胞分泌胶原的机制缺乏了解,本研究旨在观察射频对皮肤成纤维细胞的作用机制,从而更好的推进射频技术的临床运用。方法采用组织块法原代培养成人皮肤成纤维细胞,建立传代细胞系,实验用第4~6代细胞。取对数生长期的成纤维细胞,分为对照组和射频电流组,建立无创性射频除皱技术的体外细胞实验模型,应用倒置相差显微镜观察细胞形态并计数,绘制细胞生长图表,采用酶联免疫法检测细胞分泌胶原蛋白的水平。结果经射频电流作用后细胞数量明显增加,并且在一定范围内随输出电压的增加而增多,同时促进细胞分泌及合成胶原蛋白。结论射频电流刺激人皮肤成纤维细胞的生长及增殖,刺激成纤维细胞分泌胶原,是一种有效的除皱紧肤的方法。Objective To investigate on the effect of radiofrequency current on cellular proliferation and collagen secretion in human dermal fibroblast.Methods Human dermal fibroblasts from normal skin were cultured after cosmetic surgery,and the cells of 4th ~ 6th generations were used in the experiments. The proliferation effect of radiofrequency current on the FB was evaluated with inverted phase contrast microscope. The expression of collagen in supernatant of FB was detected with enzyme linked immunosorbent assay（ ELISA）.Results Radiofrequency promoted the proliferation of FB significantly and up-regulated the protein level of collagen in supernatant of FB.Conclusions Radiofrequency is an effective treatment for wrinkles.

关 键 词：射频 成纤维细胞 胶原蛋白 

分 类 号：R62[医药卫生—整形外科]