吴茱萸碱对肝癌HepG2细胞侵袭和迁徙的影响  

Evodiamine inhibits invasion and metastasis of human hepatocellular carcinoma Hep G2 cells

作　　者：孙建超[1,2] 娄方方 史云[1,2] 王豫萍[1,2] 

机构地区：[1]贵阳医学院检验学院临床微生物及免疫学教研室 [2]贵阳医学院附属医院微生物免疫科,贵州省贵阳市550004

出　　处：《世界华人消化杂志》2015年第10期1546-1552,共7页World Chinese Journal of Digestology

基　　金：贵州省省长基金资助项目;No.201244;贵州省科技厅中药现代化基金资助项目;No.20123017~~

摘　　要：目的:研究吴茱萸碱对人肝癌细胞Hep G2细胞外调节蛋白激酶(extracellular-regulated protein kinases 1/2,ERK1/2)表达的影响.方法:Transwell小室检测吴茱萸碱对Hep G2细胞侵袭及迁徙的影响.集落形成实验检测吴茱萸碱对Hep G2细胞集落形成能力的影响.Western blot检测Hep G2细胞ERK1/2、phospho-ERK1/2(P-ERK1/2)蛋白.结果:吴茱萸碱5、25、50μmol/L作用后,H e p G 2细胞侵袭细胞数分别为3 2.3个±1.3个、22.3个±2.5个、10.6个±3.7个,对照组为97.6个±6.3个,差异有统计学意义(P<0.05);迁徙细胞数为57.7个±3.2个、26.8个±1.6个、15.4个±3.9个,对照组为106.3个±3.2个,差异有统计学意义(P<0.05).5、25、50μmol/L吴茱萸碱作用Hep G2细胞集落形成数分别为147.0个±12.1个、94.3个±4.0个、0个,对照组为231.3个±15.4个,差异有统计学意义(P<0.05).Western blot分析5、25、50μmol/L吴茱萸碱处理Hep G2细胞24 h及30μmol/L吴茱萸碱处理Hep G2细胞24、48 h后,ERK1/2蛋白的表达无明显变化,但其活化形式P-ERK1/2蛋白的表达量减少.结论:吴茱萸碱可显著抑制人肝癌Hep G2细胞侵袭和迁徙及克隆增殖能力,其机制可能与吴茱萸碱抑制ERK1/2蛋白活化有关.AIM：To assess the effect of evodiamine on the invasion and metastasis of human hepatocellular carcinoma HepG2 cells and to discuss the underlying mechanism.METHODS：The effect of evodiamine on the expression levels of ERKl/2 and p-ERKl/2 in HepG2 cells was assessed by Western blot.The invasion and metastasis of HepG2 cells were tested by transwell assays.Colony-forming ability was determined by colony formation assay.RESULTS：After HepG2 cells were treated with 0,5,25,or 50 μmol/L evodiamine,the numbers of invasion cells were 97.6 ± 6.3,32.3 ±1.3,22.3 ± 2.5,and 10.6 ± 3.7,respectively,and the numbers of metastatic cells were 106.3 ± 3.2,57.7 ± 3.2,26.8 ± 1.6,and 15.4 ± 3.9,respectively（P 〈0.05）.Evodiamine had a significant inhibitory effect on HepG2 cell colony-forming ability（P 〈0.05）.Evodiamine treatment downregulated the expression of p-ERKl/2,but had no significant impact on ERKl/2 expression.CONCLUSION：Evodiamine can inhibit the invasion,metastasis and colony-forming ability of human hepatocellular carcinoma HepG2 cells,and the possible mechanism may be related to down-regulation of p-ERK 1/2 protein.

关 键 词：吴茱萸碱 HEPG2细胞 侵袭 迁徙 ERK1/2 

分 类 号：R285[医药卫生—中药学]