诱发性II型糖尿病小鼠血清细胞因子的变化  

Changes of Blood Lipid, Blood Glucose and Serum Cytokines in Different Stages of Diabetic Mice

作　　者：杨华[1] 刘芳[1] 周文江[1,2] 周晓辉[1] 任晓楠[1] 

机构地区：[1]上海市公共卫生临床中心,上海201508 [2]复旦大学药学院,上海201203

出　　处：《实验动物与比较医学》2015年第2期102-106,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的比较诱发性2型糖尿病（T2DM）小鼠6周、24周时血糖、血脂及血清细胞因子白细胞介素．6（IL．6）、白细胞介素．10（IL-10）、肿瘤坏死因子-α（TNF-α）、γ-干扰素（IFN．切水平，探讨T2DM小鼠模型的细胞免疫功能变化及其意义。方法3周龄雄性C57BL／6J小鼠46只，随机分为对照组和模型组（DM组），模型组小鼠小剂量（50mg／kg）多次腹腔注射链脲佐菌素（STZ），高脂喂养。于造模后6周、24周分别测定每组小鼠的糖耐量、甘油三酯（TG）、血清总胆固醇（CHOL）、高密度脂蛋白（HDLC）、低密度脂蛋白（LDLC）水平，同时检测血清中IL-6、IL-10、TNF-α、IFN-γ的含量。结果造模后6周，DM小鼠表现为糖耐量异常，脂代谢紊乱，血清中IL-6水平升高，TNF—α、IFN-γ、IL-10均显著高于对照组（P〈0．05或P〈0．01）。造模后24周时，糖脂代谢紊乱加重，血清中IL-6、TNF-α、IFN-γ、IL-10表达水平显著高于造模后6周水平（P〈0．01）。结论诱发性T2DM模型小鼠在成模不同时间的血糖、血脂及细胞因子的水平变化与临床类似，可作为相关研究的动物模型。Objective To compare the changes of blood lipid, blood glucose, serum cytokine interleukin - 6 （IL 6） and interleukin 10 （IL-10）, tumor necrosis factor - （TNF-α）, a gamma interferon （IFN-γ） in mice with type 2 diabetes mellitus （T2DM） during early or late stage, and discuss the mean- ings of cellular immune function changes in T2DM mouse model. Methods Fourty-six male C57BL/6 mice at the age of 3 weeks were randomly divided into control group and model group （DM group）. Animals in the model group were intraperitoneally injected with 50 mg streptozotocin（STZ）for 3 days and fed with high-fat diet. After 6 or 24 weeks injection, glucose tolerance, blood lipid （TG, CHOL, HDLC, LDLC） level and the contents of serum IL-6, IL-10, TNF-α, IFN-γ were measured and analyzed respectively. Results Six weeks after injection of STZ, abnormal glucose tolerance was observed in DM mice, with the significant higher level of lipid and serum IL-6, TNF-α, IFN-γ, IL-10 compared with normal control group （P〈0.05 or P〈0.01）. With the development of disease process, the glucose and lipid metabolic disorder aggravated in DM group after 24 weeks of injection, and the levels of serum IL-6, TNF-α, IFN-γ, IL-10 were significantly higher than those of 6 weeks （P〈0.01）. Conclusion The STZ induced mouse model showed similar disease process in terms of blood glucose, blood lipids and cytokines changes in the different courses, which might be used as the potential disease animal model.

关 键 词：诱发 糖尿病小鼠 链脲佐菌素 血糖 血脂 细胞因子 

分 类 号：R587.1[医药卫生—内分泌]