VAP血管通路在小型猪静脉糖耐量试验中的应用  

Application of Vascular-access-port in Intravenous Glucose Tolerance Test in Minipig

作　　者：陈亮[1] 潘永明[1] 蔡月琴[1] 朱科燕[1] 徐孝平[1] 陈民利[1] 

机构地区：[1]浙江中医药大学动物实验研究中心,比较医学研究中心,杭州310053

出　　处：《实验动物与比较医学》2015年第2期114-119,共6页Laboratory Animal and Comparative Medicine

基　　金：浙江省科技厅实验动物科技计划项目（2012C37078）

摘　　要：目的通过VAP（vascular—access．port）血管通路来改善小型猪静脉糖耐量试验（IVGTT）结果的可靠性，减少试验过程中的应激反应，并且为小型猪IVGTT试验提供一种便捷快速且稳定的新方法。方法将12只巴马小型猪随机分为VAP血管通路植入组与正常对照组，比较两组动物在IVGTT中的差异，并评价应激指标。结果VAP组与对照组小型猪在IVGTT试验中均能出现糖耐量反应，其血清胰岛素含量变化以及趋势无统计学差异，但VAP组血糖水平在0min，60min、90min以及120min显著低于对照组（P〈0．05，P〈0．01），且在5min血糖水平显著高于正常对照组（P〈0．01）；对照组肌酶激酶（CK）水平在各时间点均显著高于VAP组（P〈0．01）；对照组促肾上腺皮质激素（ACTH）、皮质醇（CORT）在0min、15—120min时都高于vAP组，且在60～120min时差异有统计学意义（P〈0．05，P〈0．01）。结论与常规方法比较，VAP血管通路植入后小型猪在IVGTT中所受应激更小，更符合动物福利，且试验结果更加可靠稳定。Objective To improve the reliability of intravenous glucose tolerance test （IVGTT） in minipigs and reduce the stress response during the test with vascular-access-port （VAP）, and provides a new method which was fast and convenient and stable for IVGTr in minipigs. Methods The 12 Bama mira＇pigs were randomly divided into the implantable VAP group and the normal control group, the difference of the two groups animals in IVGTT were compared, and the stress index were evaluated. Results VAP group and the control group could appear glucose tolerance test reactions in IVGTT, changes and the trend in serum insulin levels were not significantly different, but the glucose levels in VAP group at 0 min, 60 min, 90 min and 120 min were significantly lower than those of control group （P〈 0.05, P〈0.01）, and significantly higher than that of the control group in 5 min （P〈0.01）; During the test, the CK levels in the control group were significantly higher than the VAP group at each time point （P〈 0.05, P〈0.01）; ACTH and CORT levels in 0 min ,15-120 min in control group were higher than VAP group, and in 60-120 min had a significant difference （P〈0.05, P〈0.01 ） Conclusion Compare with conventional methods for IVGTT, VAP implantation way to do IVGTT suffered less stress in minipigs, more in line with animal welfare, and the test results were more reliable and stable.

关 键 词：VAP(vascular—access—port)血管通路 小型猪 静脉糖耐量试验(IVGTT) 应激 

分 类 号：Q95-33[生物学—动物学]